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Durban University of Technology (DUT) 2020

Nutritional value and bioactive properties of Opuntia ficus-indica cladodes

Mabotja, Mologadi Bernard

Titre : Nutritional value and bioactive properties of Opuntia ficus-indica cladodes

Auteur : Mabotja, Mologadi Bernard

Université de soutenance : Durban University of Technology (DUT)

Grade : Master of Applied Sciences in Biotechnology 2020

Résumé partyiel
Medicinal properties and pharmacological activities of plants have been attributed to their bioactive compounds. However, research has shown that bioactive compound concentrations, biological activities and nutritional profile are influenced by cultivar type. The aim of the study was to characterize the cladodes of 42 spineless cultivars of Opuntia ficus-indica at the Agricultural Research Council in terms of their chemical, nutritional and medicinal properties. The antidiabetic potential of selected extracts was investigated in vitro against alpha-glucosidase enzyme. Aqueous methanol extracts were assayed for total phenolic and flavonoid content, and antioxidant activities using 1,1-Diphenyl-2- picrylhydrazyl (DPPH) and β-carotene linoleic acid system assays. Antibacterial activities of different extracts were assessed against two Gram (-) and two Gram (+) bacteria and their minimum inhibitory concentration (MIC) values recorded. Vitamin C, β-carotene and individual phenolic acid contents were analysed using a Shimadzu HPLC (LC-2030C 3D) equipped with a C18 Luna® column. Quantification was achieved by calibration curve plotted using different standards. Mineral elements were quantified using inductively coupled plasma - optical emission spectrometry. There were variations in the yield depending on the cultivars, however, 50% methanol extracts generally had higher yield as compared to petroleum ether extracts. Significant variation in phytochemical composition, pharmacological activities and nutritional value was observed in the different cultivars studied.

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