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Instituto Politécnico de Bragança (2021)

Quality evaluation of Algerian honeys : Eucalyptus, Jujube, Euphorbia and multiflora

Kaid, Seloua

Titre : Quality evaluation of Algerian honeys : Eucalyptus, Jujube, Euphorbia and multiflora

Auteur : Kaid, Seloua

Université de soutenance : Instituto Politécnico de Bragança

Grade : Master in Biotechnological Engineering 2021

Résumé partiel
This study was intended to evaluate the quality parameters of ten honey samples, from various regions in semi-arid region of Algeria. Different parameters such as the melissopalynological and the physicochemical properties of the honeys (moisture, color, electrical conductivity, 5-hydroxymethylfurfural, pH, acidity, proline, and diastase activity) were determined, as well as the evaluation of sugars, minerals and phenolic compounds. Nutritional composition, antioxidant activity (reducing power and DPPH free radical scavenging activity), anti-inflammatory and cytotoxicity were also evaluated. Finally, antibiotics residues such as sulphonamides and tetracyclines antibiotics residues were screened. The melissopalynological results identified ten types of pollen, with Cytisus striatus pollen being identified as the most abundant, present in all samples with percentages between 26.0 % and 83.8 %. EC1, MF1 and MF2 (Sidi Belabes region) were classified as monofloral of Cytisus striatus honey. Additionally, although samples J1 to J3 were not considered as Jujube monofloral, they showed a high percentage of Ziziphus pollen. The remaining samples (EC2, EF1, EF2, and EF3) were classified as multifloral. Regarding the physicochemical parameters, no significant differences were found in the color of the samples which ranged between amber, light amber and extra light amber. Moisture content was found to be between 13.6% (EF1) and 18.3% (EC1), while pH values ranged between 4.2 and 5.1. Electrical conductivity values varied between 270 and 410 μS.cm-1, while 5- hydroxymethylfurfural content showed values between 0 and 36.5 mg.kg-1 and diastase values between 8.8 DN and 13.3 DN. Concerning the proline content, the samples showed proline levels between 2.2–4.7 mg/kg, indicating a good maturity of the honeys and absence of adulteration. All the honeys meet the standard required by the European legislation with exception of the diastase index. The sugar profile, analyzed by high pressure liquid chromatography with refractive index detection (HPLC-RI), showed that all samples have higher fructose content than glucose, being the total more than 88.70 %, allowing the classification of all the samples as nectar honeys.

Mots clés  : Honey Melissopalynological analysis:Physicochemical parameters Antioxidant activity Anti-inflammatory activity Cytotoxicity Antibiotic residues

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