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King Abdulaziz University (2020)

MOLECULAR AND BIOCHEMICAL STUDIES ON SOME HALOPHYTES IN THE COASTAL AREA OF SOUTH JEDDAH

Al-Mushdq, Amina Saad

Titre : MOLECULAR AND BIOCHEMICAL STUDIES ON SOME HALOPHYTES IN THE COASTAL AREA OF SOUTH JEDDAH

Auteur : Al-Mushdq, Amina Saad

Université de soutenance : King Abdulaziz University

Grade : Doctorate Thesis 2020

Résumé
Halophyte species of Saudi Arabia are a source of unique active phytochemicals, which enable them to withstand stress conditions and are involved in resident adaptation, potentially due to the extreme environmental conditions under which the plants grow in the KSA. The current study concerned with seven halophytic species,that collected from different localities, along south Jeddah coast. In addition, Roots, leaves and flowers/fruits samples from all collected species were screened by GC-MS to identify the most abundant phytochemical constituents. Ultimately, depending on the results of the phytochemical analysis, four species were selected for RT-PCR analysis in order to verify the relative gene expression of four selected salt-tolerant genes. Depending on GC-MS analysis, 50 phytochemical compounds were identified in each plant extract. These compounds predominantly classified as alkaloids, terpenoids, flavonoids, phenols, steroids, alkanes, alcohols, fatty acids, esters and organic acids. S. monica and A. marina, that grow in the highest saline conditions, possess the highest levels of many active phytochemicals, mainly those belong to alkaloids and terpenoids, compared to T. coccinea, A. lagopoides, T. alba and T. simplex. Numerous of the identified compounds are bioactive phytochemicals and proved to hold a broad range of activities. Hence, from this study it can be confirmed that these species could be used as a new potential source for new drugs .RT-PCR analysis demonstrated significant differences in , GLR, PDI and GRP genes expression level in the leaves of the selected studied species. In general, significant higher expression level of all investigated genes was noticed in S. monoica and A. marina as compared to H. perfoliata and A. lagopoides, which indicated higher adaptability in the species that grow in conditions that are more saline.

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Page publiée le 14 décembre 2022