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King Abdulaziz University (2020)

Ecological and molecular Studies on the fish Aphanius fasciatus (valencienns, 1821) from valley Dam Ganona, Saudi Arabia

Al-Harthi, Ayed Mohammed

Titre : Ecological and molecular Studies on the fish Aphanius fasciatus (valencienns, 1821) from valley Dam Ganona, Saudi Arabia

Auteur : Al-Harthi, Ayed Mohammed

Université de soutenance : King Abdulaziz University

Grade : Master Thesis 2020

Résumé
Fresh water in Saudi Arabia is very scarce, so dams were set up on waterways to reserve rainwater and prevent it from flowing into the sea, so it can use it in agriculture and drinking for animals. The rainwater may be polluted as it passes through polluted areas and soils. The Wadi Qnona in the North in Al-Aradia province of the south west of the Kingdom following Makkah region, is one of these dams in the Kingdom of Saudi Arabia. The results of physical and chemical characters of water showed, that its electrical conduction EC and turbidity is less that local of international standards valueo. Water is low in water hardness, and good terms of total dissolved salts (TDS), dissolved oxygen content (DO), NH4 ammonia, nitrite NO3 and NO2 are low and far less than FAO standard (FAO 1985) and the Saudi Ministry of Water and Electricity (MWE 2005). Sulphates (SO4) content is lower than WHO standard and the standard Europe countries. For HCO3 and Cl2 concentrations, the standards are lower than the global standards for drinking water and agriculture. As for the dam water content of major elements Na, Mg, Ca and K the concentrations are all below the standards set locally and internationally for drinking water and agriculture. The water content of heavy and toxic metals (Fe, Mn, Cu, Zn, Alcd, Cr, Ni, Pb, Co, Hg, Ti, Ag, Ar, Mo, Sb, Be, V, As and Ba) are very low in comparison with local and international standards set for the concentrations of these minerals in water used for drinking or agriculture because the high concentrations of these elements in water is one of the most serious problems. Based on the results of this study we recommend the use of water dam Wadi Qnona for agricultural purposes and to drink animals, they are valid and do not cause health damage. Currently, DNA barcoding, especially the mitochondrial cytochrome oxidase subunit I (COX-I) gene is highly recommended as an accurate method to identify animal species. So, this method is used to identify the fish species in the present study. The obtained results reveal that COX-1 gene of this fish species composed of about 700 bp, and their sequence was compared to the NCBI GenBank® sequence database. This reveals that COX-1 sequence in the present fish species was closely related (98% identity) to that of Aphanius fasciatus. This confirms, and without doubt, that the fish studied in this research is belonging to thi species.

Présentation

Page publiée le 18 janvier 2023