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Titre : Phytochemical and Biological Study of Euphorbia cuneata

دراسة كيميائية وبيولوجية لنبات يوفوربيا كيونات

Auteur : Alghamdi, Amjad Ahmed

Université de soutenance : King Abdulaziz University

Grade : Master Thesis 2020

Résumé
The aim of current study was to isolate and identify active metabolites of Euphorbia cuneata (family Euphorbiaceae) and assess their cytotoxic activity on three different cell lines : MCF-7 (breast cancer), HepG2 (hepatocellular carcinoma), and HCT-116 (colon cancer). Phytochemical study of the methanolic extract of Euphorbia cuneata aerial parts using different chromatographic techniques led to the isolation of two new compounds : named cyclocuneatol (2) and cuneatannin (11), along with nine known metabolites : β-sitosterol (1), 3β-hydroxycycloart-25-ene-24-one (3), cycloart-25-ene-3β,24β-diol (4), cycloart-23Z-ene-3β,25-diol (5), 2R-naringenin (6), β-sitosterol-3-O-β-D-glucopyranoside (7), quercetin (8), kaempferol-7-O-β-glucoside (9), and 2R-naringenin-7-O-β-glucoside (10). Their structures were established on the basis of physical, chemical and spectral data (UV, IR, 1D (1H and 13C NMR) and 2D NMR (1H-1H COSY, HSQC, HMBC and NOESY), as well as HRESIMS and comparison with literature data. Their cytotoxic activity was assessed towards HepG2, MCF7, and HCT116 cancer cell lines by sulphorhodamine B test (SRB). It is noteworthy that 3 and 4 demonstrated the most significant activity towards HepG2, MCF7, and HCT116 cells (IC50 2.6, 2.7, and 2.4 μM, respectively for 3 and 3.4, 4.1, and 5.3 μM, respectively for 4) in comparison with doxorubicin (IC50 0.18, 0.6, and 0.2 μM, respectively).

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