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Botswana University of Agriculture and Natural Resources (BUANR) 2022

Assessment of genetic diversity of mungbean genotypes in Botswana using agro-morphological traits and molecular markers.

Kgokong, Sylvia Boineelo

Titre : Assessment of genetic diversity of mungbean genotypes in Botswana using agro-morphological traits and molecular markers.

Auteur : Kgokong, Sylvia Boineelo

Université de soutenance : Botswana University of Agriculture and Natural Resources (BUANR)

Grade : Master of Science in Crop Science (Agronomy) 2022

Résumé partiel
Mungbean (Vigna radiata L, Wilczek), is one of the leguminous crops that plays an important role in improving national food security and sustaining livelihoods of small-scale farmers in the developing world. Despite its economic importance, it has not been fully exploited in Botswana due to insufficient or lack of information on genetic background of the cultivated landraces. Therefore, the main objective of this study was to evaluate genetic diversity of eighteen mungbean genotypes in Botswana using agro-morphological characters and molecular markers. Two experiments were conducted in greenhouse and field conditions and laid in a randomized complete block design (RCBD) with three replications. A total of 34 quantitative and 11 qualitative traits were assessed. The qualitative morphological data revealed variation among traits including ; growth habit, leaf colour, raceme position, pod curvature, pod colour, seed colour and testa texture. Analysis of variance (ANOVA) showed significant differences (P ≤ 0.05) in yield and yield components, photosynthetic rates, chlorophyll content and root characteristics among the genotypes. The relationships among the tested traits of the genotypes were identified by Pearson’s correlation and principal component analysis and the first four principal components accounted for 66.7% of the total variation. NTSYS-pc software using Unweighted pair group method with arithmetic mean (UPGMA) generated a dendrogram that distinctly grouped the 18 genotypes into four major clusters based on qualitative and quantitative morphological traits. For molecular analysis, 15 SSR makers were identified and used to assess genetic diversity among mungbean genotypes. Polymorphic information content (PIC) values ranged between 0.23 and 0.99 with highest value observed in CEDG305 and DMB-SSR 182 and the allele number per locus varied from 1 to 3 with maximum allele number produced by primer LR7323B and CEDG264. The vi dendrogram constructed based on the SSR markers through UPGMA grouped the genotypes into seven clusters and the grouping of genotypes was not related to their geographic origin.

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Page publiée le 23 décembre 2022