Informations et ressources scientifiques
sur le développement des zones arides et semi-arides

Titre : Características espermáticas de cutias (Dasyprocta leporina Linnaeus, 1758) durante o trânsito epididimário e nas estações seca e chuvosa do bioma caatinga

Auteur : Dantas, Maiko Roberto Tavares

Université de soutenance : Universidade Federal Rural do Semi-Árido (UFERSA)

Grade : Doutorado em Ciência Animal 2022

Résumé partiel
Since the reproductive characteristics of wild rodents are affected by environmental factors, understanding the seasonal influence on the sperm characteristics of agoutis during sperm maturation is essential for the conservation of this and other phylogenetically related species. Therefore, the objective was to evaluate the effects caused by the climatic conditions of the dry and rainy periods in a semiarid region on the morphofunctional sperm parameters of agouti (Dasyprocta leporina) during epididymal transit. Therefore, the study was divided into three experiments, using adults animals from the Center for Multiplication of Wild Animals of UFERSA. In experiment I, the objective was to describe the ultrastructural, kinetic, morphological, and morphometric changes of agouti sperm during epididymal transit, including its maturation. For this, seven testis-epididymis complexes of sexually mature agoutis were removed, and sperm from different regions of the epididymis (caput, corpus, and cauda) were collected using the flotation technique. There were no significant differences between epididymal regions and normal sperm morphology. The mean head length, head width, and tail length of sperm were higher in the epididymal caput (5.15 μm, 3.44 μm, and 32.04 μm, respectively), decreasing along the epididymal transit. Spermatozoa from the cauda of the epididymis showed the highest values of membrane integrity with mitochondrial activity (79.71%). There were significant increases in total motility and several kinetic parameters following the caput-corpus-cauda direction. We provide unprecedented data related to epididymal transit of sperm in agoutis

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