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New Bacterial Culture Methods Could Result in the Discovery of Diverse New Species of Microbes from the Tabernas Desert

SciTechDaily (JANUARY 16, 2021)

Titre : New Bacterial Culture Methods Could Result in the Discovery of Diverse New Species of Microbes from the Tabernas Desert

The culture-dependent study allowed to isolate a surprisingly large number of diverse and previously unreported bacterial strains from the Tabernas Desert.

SciTechDaily (JANUARY 16, 2021)

Présentation
Microorganisms are the most abundant and diverse form of life on Earth. However, the vast majority of them remain unknown. Indeed, only a small fraction of the microorganisms of our planet can be cultured under traditional conditions, leaving a world of unculturable organisms out of our scope. This is especially true for bacteria thriving under extreme conditions as the harsh conditions are hardly reproducible in a lab. While some microbial studies have been performed in the Sahara, the Atacama, and the Gibson desert, European arid lands remain poorly studied. To finally explore the microbial community of some European deserts, researchers from the University of Valencia and the Darwin Bioprospecting Excellence, here studied the bacterial diversity of the semi-arid Tabernas Desert. To this aim, the team developed new bacterial culture approaches.

The researchers experimented with different culture methods to find permissive conditions for some unculturable species. Their strategy lied in combining different media, using serial dilutions of the nutrients (up to a hundred times), and extending incubation time (up to a month). In total, 254 bacterial strains were isolated. Most of the species isolated from the concentrated media were previously described as soil inhabitants or species isolated from other deserts. However, 60% of the strains isolated from the highly diluted media are non-identified and possibly new bacteria species. Besides, playing on incubation times also allowed, after a month, to isolate some oligotrophic strains (slow-growing bacteria living under low nutrient conditions) otherwise difficult to growth under lab conditions.

Source  : FRONTIERS

Annonce (SciTechDaily)

Page publiée le 17 janvier 2023