Informations et ressources scientifiques
sur le développement des zones arides et semi-arides

Titre : Phytochemical and anticancer studies on Bougainvillea spectabilis, Alhagi graecorum, Retama raetam and Psoralea plicata growing in Libya

Auteur : Atraiki, Rajab

Université de soutenance : University of Strathclyde

Grade : Doctor of Philosophy (PhD) 2021

Résumé partiel
This thesis examined the in vitrophytochemical and cytotoxicity/anticancer activities of four selected plants from the Libyan flora. Based on information on traditional medicinal uses and a literature survey, Bougainvillea spectabilis Willd. (Fam : Nyctaginaceae), Alhagi graecorum Boiss (Fam : Fabaceae) Retama raetam (Forssk.) Webb & Berthel. (Fam : Fabaceae), and Psoralea plicata (Fam : Fabaceae) were selected. The four plants were investigated phytochemically and a range of compounds were elucidated, including : pinitol, allantoin, trigonelline, narcissin, alpinumisoflavone, 8-β-D-glucopyranosylgenistein, ephedroidin, plicatin-A, psoralen, angelicin and daidzein. The study concentrated on the evaluation of n-hexane, ethylacetate (EtOAc),methanol (MeOH) and ethanol (EtOH) solvent plant extracts and some of the isolated compounds for cytotoxicity based on metabolic activity to evaluate the potential anticancer activity against six human tumour cell lines : human Caucasian hepatocyte carcinoma (HepG2), human malignant melanoma (A375), human Caucasian lung carcinoma (A549), human Caucas ian pancreas carcinoma (PANC-1), human Caucasian breast adenocarcinoma (MCF7), human Caucasian prostate carcinoma (LNCaP), and non-cancerous human normal prostate (PNT2) cells as the normal control. Cytotoxicity at different concentrations (1.95, 3.90, 7.81, 15.62, 31.25, 62.5, 125 and 250μg/ml) of these extracts was evaluated using a resazurin assay. B. spectabilis extracts and its isolated constituents pinitol (Bs-1), allantoin (Bs-2) and trigonelline (Bs-3) showed no cytotoxic activity on any of the cell lines.

Présentation

Version intégrale