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Shoolini University of Biotechnology and Management Sciences (2021)

Chemosystematic investigation of Juniperus diversity in cold desert of Himachal Pradesh

Chauhan, Vijay

Titre : Chemosystematic investigation of Juniperus diversity in cold desert of Himachal Pradesh

Auteur : Chauhan, Vijay

Université de soutenance : Shoolini University of Biotechnology and Management Sciences

Grade : Doctor of Philosophy (PhD) 2021

Résumé
The genus Juniperus is widely distributed in the north hemisphere (cold desert) and well known source of cedar wood oil as well as folk medicine. Juniperus has been reported as diuretic, anti-inflammatory, analgesic, hepatoprotective, antidiabetic, antihyperlipidemic, antimicrobial, antioxidant, antihypercholesterolemic, anticataleptic and neuroprotective activity. Keeping in view the above traditional and medicinal properties, three important species of Juniper (J. communis, J. macropoda and J. recurva) were collected from three different regions of cold desert of north western Himalayas of Himachal Pradesh (Kalpa, Chitkul and Pooh). The studies were conducted taking into account the different parameters like physicochemical characterstics of soil, biochemical analysis of leaves and berries and quantitative determination of bioactive components by GCMS in essential oil and HPLC in extracts respectively. Further chemometric analysis was carried forward to assist the significance and structural analysis using scatter plot(SP), principle component analysis (PCA), discriminant analysis (DA), and hierarchical cluster analysis(HCA). The results obtained for the above incorporated methodologies displayed a positive correlation between soil and biochemical analysis of berries and leaves samples with increasing altitude. Quantification of secondary metabolites from essential oils showed maximum yield (2.08%) in berries sample of J. recurva and minimum (1.26%) was recorded in leaves samples of J. macropoda. In addition, monoterpene hydrocarbons like β phellandrene, β-pinene, 3-Carene, α-Pinene, Limonene and Sabinene were dominant in leaves and berries as compared to the sesquiterpenes (hedycaryol, α-humulene, (E)-β Farnesene, (+)-germacrene-D, α-himachalene etc). The studies further summarize that higher rutin and quercetin content (39.49 µg/ml and 12.65 µg/ml) was observed in berries of J. communis and minimum was reported in the leaves J. macropoda (rutin 5.70 µg/ml and quercetin 1.07 µg/ml). Chemometric analysis of the data obtained from GCMS indicates 3-Carene as the most dominant compound with respect to PCA and DA. Four clusters were observed in the hierarchical cluster analysis specifying closely related linkage between J. macropoda and J. recurva whereas ; J. communis was identified with a large diverging pattern from the other two species. The study finally explored the potential related to Juniper in terms of healthcare and industrial applications and can be further utilized for the industrial purposes, cosmetics, health sector and pharmaceutics.

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Page publiée le 21 janvier 2023