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sur le développement des zones arides et semi-arides

Titre : Effect of Dietary Supplementation with Probiotic and Prebiotic on Physiological Performance and Rumen Ecology of Growing Lambs

Auteur : Walieldin Elsadig Elmgboul Hamad

Université de soutenance : University of Khartoum

Grade : Doctor of Philosophy in Veterinary Science (Physiology) 2022

Résumé partiel
The objectives of this study were to evaluate the effects of dietary supplementation with probiotic (BIOGEN B) and prebiotic (Biolex®MB40) on growth performance, physiological responses, rumen ecology and histometric measurements of the small intestine in lambs under three different feeding regimes. Sixty three male desert lambs (Hamari ecotype, 5 month) were obtained from the local livestock market. The animals were allowed to adapt for 2 weeks. Lambs were kept in naturally ventilated, open-sided animal house. Oxytetracycline L.A 20%, Ivermectin and Albendazole were administered during the adaptation period. The study was performed at the Department of Physiology, Faculty of Veterinary Medicine, University of Khartoum (during February 2020 to March 2021). The study was conducted in three separate experiments. The experiment was performed in a CRD. In each experiment, 21 lambs were assigned to 3 equal groups (A, B and C). In experiment I, lambs were fed roughages Rhodes grass (Chloris gayana) ad libitum and concentrates (0.4 kg /animal/ day), in experiment II, roughages alfalfa (Medicago sativa) ad libitum and in experiment III, roughages alfalfa (Medicago sativa) 50% feed restricted. Group A served as a control, Whereas group B supplemented with probiotic (0.5 g /animal/ day) and Group C supplemented with prebiotic (3 g/ animal/ day). The duration of each experiment was 8 weeks. The BW and BWG were recorded every two weeks, blood samples were collected weekly. Haematological parameters assessed were PCV, Hb, TEC and TLC. plasma glucose (Gl), and serum concentrations of total protein (TP), albumin (Alb), globulins (Glo), cholesterol (Chol), urea (Ur), creatinine (Cr), ALT, AST, Na+ , K+ , Clˉ, Ca+2 and Mg+2, and thyroid hormones (T3 and T4) were determined. Rumen liquor samples were collected every two weeks for monitoring pH, TVFA and NH3. Samples from duodenum, jejunum and ileum were collected at the end of each experiment for histometric measurement. SPSS V.23 was used for data analysis using one way ANOVA test.

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