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sur le développement des zones arides et semi-arides

Titre : Pharmacological and Chemical Profiling of Three Selected Sudanese Medicinal Plants Based on a Battery of Isolated Organs Coupled with LC-MS Guided Fractionation

Auteur : Rayan Hassan Mohamed Elshikh Idriss

Université de soutenance : University of Khartoum

Grade : Master of Pharmaceutical Sciences (Pharmacology) 2022

Résumé partiel
Recent advances in both biochemical screening methods, and modern phytochemical techniques provided an extra impetus to drug discovery of bioactive molecules from medicinal plants. The aim of this research work is to investigate the pharmacological activity and identify the main chemical constituents associated with the bioactivity of three selected locally used medicinal plants namely, Solenostemma argel Hayne, Haplophyllum tuberculatum (Forssk.) A. Juss, and Sonchus oleraceus L. Methods : All three medicinal plants were extracted with 70% ethanol, concentrated, and fractionated with n-hexane, chloroform, ethyl acetate and n-butanol, sequentially. Extracts and fractions were screened pharmacologically, and bioactive fractions were subjected to high performance liquid chromatography coupled to tandem mass spectrometry (LC/MS/MS). Results : Crude extract of S. argel leaves reduced the force of contraction which was measured in terms of length of the QRS complex in a dose dependent manner (1.7-0.5cm for the highest dose) compared to control length (2cm) while the chloroform fraction stimulated the intestine and pregnant uterus (2.9cm for the highest dose compared to 1.5cm of the control). Meanwhile, the aqueous residue stimulated the non-pregnant uterus. LC-MS-MS analysis of S. argel fractions revealed presence of the 14,15α-dihydroxy progesterone in both the n-hexane and chloroform fractions whiles the n-butanol fraction contained mainly flavonoid glycosides. H. tuberculatum roots chloroform fraction exhibited relaxant effect on pregnant uterus in terms of contraction peak length (3-0.3cm) while all other fractions except chloroform exhibited relaxant effect on non-pregnant uterus. The crude extract of its aerial parts revealed relaxant effect on non-pregnant uterus with increase of force of contractions on the rabbit heart (0.3-1.3cm for the highest dose).

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