Informations et ressources scientifiques
sur le développement des zones arides et semi-arides

Titre : Production of some wheat lines resistant to some unfavorable conditions

Auteur : Eigarhy, Hoda Aly Salem.

Etablissement de soutenance : Benha University .

Grade : Doctor of Philosophy in Agriculktural Science 2005

Résumé
This study aimed to introduce a stress-related gene in order to improve tolerance of one Egyptian wheat cultivar (Giza 164) to oxidative stress, i.e., drought and salinity. This goal was achieved by introducing plant expression vector pLe-GST Thar harboring one of oxidative stress tolerance-related genes namely Le-GST, Lycopyrsicon esculuntium glutathione s-transferase, that generates a signal for scavenging and detoxification of the highly reactive oxygenated compounds (ROS) into the genome of the cultivar (Giza 164) using immature embryo-derived calli as a target tissue and microprojectile bombardment as a transformation device. However, the Le-GST gene was driving by CaMV 35S promoter. Plant expression vector pLe-GST/bar also contained bar gene as a selectable marker for the herbicide bialaphos resistance.

Présentation étendue (EULC)