Informations et ressources scientifiques
sur le développement des zones arides et semi-arides

Titre : An attempt to reduce drought resistant plants using biotechnology methods

Auteur : Rizkalla, A. A.

Etablissement de soutenance : Benha University .

Grade : Doctor of Philosophy in Genetics 2000

Résumé
This work is an attempet to produce plants can resist salt stress and also drought stress, two screening strategies can be applied using T- DNA mutants from Arabidopsis ecotype Wassilevskija using the binary vector pGKB5 which possesses the reporter GUS promoterless gene from Versailles lines INRA. Screening for. 44 pools of 100 with two strategies permitted obtaining of Arabidopsis putative mutants can resist high salt concentration (300 111M : NaCl) . • The first strategy (loss -of - function screening) :- This strategy depending on isolating mutants, their genomes, were interrupted by the GUS promoterless gene that resulting in loosing the function of these genes (susceptibility to salt stress). 44 pools of 100 from Versailles lines were screened with selection system for salt resistance in addition to the pools of 20 and their individuals which gave positive results. We set up this screening with wild type and the chosed lethal concentration was 300 mM which kills the seedlings of wild type completely after 15 days. The survival seedlings which can grow and exhibited vigorus growth after the death of the wild type as a control are considered survivals (putative mutants). Two mutant lines were obtained through this screening from pools of 20 (N5308-1 and N5308-2), two Kan. mutants resistant, germinated on Kan. (50pg/ml) with the ratio 3-1, it means that these mutants have one copy of integrated T - DNA .So through screening of 44 pools of 100, and pools of20 which belong to 15 pools of 100 gave positive results, we could isolate two mutant lines that can resist salinty / drought stress.

Présentation étendue (EULC)