Informations et ressources scientifiques
sur le développement des zones arides et semi-arides

Titre : ENHANCEMENT OF ARTEMISININ PRODUCTION from SERIPHIDIUM HERBA- ALBUM USING BIOTECHNOLOGICAL TOOLS

Auteur : Aboelhasan, Fatma Mohamed Omran.

Etablissement de soutenance : Alexandria University

Grade : Doctor of Philosophy (PhD) in Genetics 2021

Résumé partiel
The experiments of the present study were conducted in the Plant Tissue Culture and Biotechnology Labs, Maryuot Research station, Desert Research Center, Egypt and the Department of genetics, Faculty of Agriculture, Alexandria University, Alexandria, Egypt. Seriphidium herba-album is very important medicinal plants that have been used in folk medicine for many years till now for the treatment of gastric disturbances and healing external wounds, remission of diabetic symptoms, activating of the liver and healing rashes, joint pains, inflammations and rheumatoid arthritis, with no side-effects. S. herbaalbum belongs to Artemisia genus, family of Asteraceae which contains about 23000 species. Artemisia genus plant produces a various number of secondary metabolites that showed biological activity. Artemisinin is sesquiterpene lactones. This substance is known for its medical efficacy, antioxidant, strong anti-inflammatory, antitumor activity, antimalarial, and for the fact Artemisinin could increase immunity. The results : 1. Establishment of a regeneration system for (Seriphidium herba-album) 1.1. The highest percentage of Culture asepsis in the stem nodal section explants was (90%) with (73.33%) survival. This result was obtained when used 25% of sodium hypochlorite (Clorox) for 20 minutes then soaking in 0.1 % mercuric chloride (HgCl2) for five minutes 1.2. The highest callus induction percentage (97.44%) and callus fresh weight (7.89 gram) were obtained on MS medium supplemented with 1.5 mg/L 2, 4-D and 0.5 mg/L BAP after four weeks from callus culture 1.3. The highest percentage of somatic embryos formation (95.30%) and the average number of somatic embryos per 1.0 g fresh weight of embryogenic callus (86.38) wre obtained on MS medium supplemented with 5.0 mg/L BAP and 0.5 mg/L NAA compared to the other treatments after eight weeks from callus calture 1.4. The best results of somatic embryo germination percentage (89.75%) and mean shoot length (3.98 cm) of the Seriphidium herba-album were obtained on MS medium supplemented with 3.0 mg/L GA3 and 1.0 mg/L kinetin compared to the other treatments after eight weeks from callus calture 1.5. The highest percentage of shoots that forming roots reached value of 100% and the highest mean number of roots/shoot (25.0%) and the highest mean length of roots/shoots (9.0 cm) were on MS medium free-hormone 1.6. The survival rate 100% after four weeks from the removal of transparent bags plantlets were maintained successfully. It decreased to 97% after 10 weeks of acclimatization 73

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