Informations et ressources scientifiques
sur le développement des zones arides et semi-arides

Titre : Molecular characterization of alpha subunit of sodium pump gene and its relation to energy metabolism in Camelus dromedarius /

Auteur : El-Kassas, Ahmad Hamdy Kamal.

Etablissement de soutenance : Cairo University

Grade : Doctor of Philosophy (PhD) 2022

Résumé
Camelus dromedarius is uniquely adapted to survive the desert environment. It has an excellent ability to compete with dehydration with significant tolerance. Therefore, it offers an excellent model for studying osmoregulation. Molecular characterization of Na+/K+ ATPase as an integral regulator of electrolyte normohemostasis affords a better understanding of this mechanism in camel. Here is the first report to resolve the full-length sequence of alpha-1 subunit of the sodium pump (ATP1A1) gene in the dromedary camel. The recovered full cDNA of ATP1A1 was submitted to the GenBank (NCBI GenBank accession #MW628635) and bioinformatically analyzed. The cDNA sequence revealed 3760 bp with an open reading frame (ORF) of 3066bp encoding a putative 1021 amino acids polypeptide with a molecular mass of 112696Da. Moreover, different organs’ transcriptomic screening was performed by real-time PCR. The results confirmed a direct function between the ATP1A1 gene expression and the vital performance of the target organs. The transcript levels of ATP1A1 showed marked upregulation in both the adrenal gland and brain compared to the other studied organs and so reflecting the role played by the Na+/K+ ATPase in both organs.

Présentation étendue (EULC)