Informations et ressources scientifiques
sur le développement des zones arides et semi-arides

Titre : Desert Wild Edible Truffles (Terfezia spp.) and the associate microbiota as sources of bioactive agents

Auteur : Sallam, Fatma Ebrahim Abdel Zaher Hefny.

Etablissement de soutenance : Ain Shams University

Grade : Master of Science Microbiology 2022

Résumé partiel
The steps of study were conducted as following :  Two types of truffles, both premature and mature, were collected from Al-Hammam City in Marsa Matrouh in the Western Egyptian Desert (during January and March 2019)  In vitro :  Phenotypic characterization of white and brown truffles  Genotypic characterization for truffles (18S rRNA)  Constituent analysis for both type of desert truffles  Qualitative of some enzymatic activity of truffles  Protein profile of mature truffles  Antimicrobial activity of premature and mature truffles  TEM micrographs of pathogens  Antitumor activity of mature truffles  Evaluation of antioxidant activity of mature truffles using DPPH scavenging  In vitro antidiabetic activity of mature T. nivea and T. canerienasis (α-Amylase inhibition)  Isolation, purification and characterization of culturable truffle -associated microbiota  16S r RNA identification of selected isolates.  Phenotypic characterization of fungal isolates.  Using bioinformatic analysis : phylogenetic tree and distance matrix, consensus, and SNP clustering analysis studied the correlation between truffles and identified bacterial isolates.  Statistical analysis

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