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Accueil du site → Master → Etats Unis → 2011 → Investigating the mechanical, physiological and molecular characteristics in drought resistant and drought susceptible Eragrostis species

Villanova University (2011)

Investigating the mechanical, physiological and molecular characteristics in drought resistant and drought susceptible Eragrostis species

Xiang, Wenxi

Titre : Investigating the mechanical, physiological and molecular characteristics in drought resistant and drought susceptible Eragrostis species

Auteur : Xiang, Wenxi

Université de soutenance : Villanova University

Grade : Master of Science (MS) 2011

Résumé
My research focused on understanding the underlying mechanicalproperties and the role of the important signal transducer 14-3-3 group of proteins in drought-tolerant and desiccation-tolerantEragrostis species in their response to drought and desiccation tolerance. A closely related species Sporobolus staphianus (desiccation tolerant) was used as an out group. We conducted a series of experiments including tensile strength, absolute water content, water potential, electrolyte leakage, light microscopy to ascertain any potential differences in the anatomy, morphology and mechanical properties. Western blot analyses was used to study changes in the expression of eukaryotic signal transducer 14-3-3 proteins under drought stress in this genus. Since the 14-3-3 fromEragrostis is not known, primer walking experiments were carried out to compare the conserved cDNA regions to the known Arabidopsis thaliana 14-3-3 cDNA conserved sequence. My research data supports previous studies that demonstrated that drought anddesiccation tolerant grasses utilize different survival strategies during water deprivation. We suggest that 14-3-3 proteins play a role in lignin biosynthesis which in turn contributes to plantdrought resistance. From my studies, the drought resistance mechanisms of Eragrostis viscosa are shown to be independent of lignin deposition but instead related to high osmotic potential.

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Page publiée le 13 mai 2012, mise à jour le 19 novembre 2018