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Andhra Pradesh Agricultural University (1982)

Identification and characterization of cucumber mosaic and bean yellow mosaic viruses affecting Chickpea (cicer arietinum.L.) in India

Chalam, T V

Titre : Identification and characterization of cucumber mosaic and bean yellow mosaic viruses affecting Chickpea (cicer arietinum.L.) in India

Auteur : Chalam, T V

Etablissement de soutenance : Andhra Pradesh Agricultural University.

Grade : PhD thesis 1982

Résumé partiel
Two mechanically transmissible viruses ; viz., cucumber mosaic virus (CMV) and bean yellow mosaic virus (BYMV) were found to naturally infect chickpeas. These were identified by studying their host range, biophysi cal properties, insect transmission, electron microscopy and serology. Also elite chickpea germplasm accessions were screened for resistance to these viruses. Both the viruses initially produced twisting of terminal bud in chickpea. Subsequently, CMV either produced wilting and death of plants or proliferation and bushiness of branches bearing very small green leaves and very small pods, whereas BYMV produced proliferation of branches with narrow or filiform leaves, bearing very small pods. The (51V could infect 13 plant species belonging to five families, whereas the host range of BYMV was restricted to Leguminosae only. The results on in vitro properties indicated that CMV was more stable than BYMV. Both the viruses could be transmitted by two aphid species ; viz., Aphis maccivora and t$jzus perskae in a non-persistent manner. An efficient purification procedure developed for CMV yielded 80-100 mg of virus per kg of plant tissue. The CMV particles were spherical and measured about 30 nm in diameter. The purified CMV had a buoyant density and sedimentation coefficient of 1.325 g/ml and 104S, respectively. Using the polyacrylamide gel electrophoresis (PAGE), the molecular weight of CMV coat protein was determined to be 25,000 daltons. The PAGE of CMV WAS revealed the presence of four RNA species, The present strain of CMV was relatively more closely related to CMV-C pool, followed by CMV-Ix and M-CMV. The antiserum produced to CMV had a titre of 1/1024 as determined by agar double-diffusion test.

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