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Doctorat
Inde
2010
Study of inheritance and identification of molecular markers for fertility restorers in pigeonpea [Cajanus cajan (L.) Millspaugh]
Titre : Study of inheritance and identification of molecular markers for fertility restorers in pigeonpea [Cajanus cajan (L.) Millspaugh].
Auteur : Saxena, R K
Etablissement de soutenance : International Crops Research Institute for the Semi-Arid Tropics .
Grade : PhD thesis 2010
Résumé
An effort has been made to understand the genetics of fertility restoration (Fr) and linking molecular markers with this trait in pigeonpea. A set of 159 A-, B- and R- lines were screened for polymorphism with 148 simple sequence repeat (SSR) markers to choose the most diverse crossing parents. In total, 41 SSR markers showed polymorphism with 2 to 6 alleles and 0.01 to 0.81 polymorphism information content value across the lines surveyed. Two parental combinations for each early (ICPA 2039 × ICPR 2438 and ICPA 2039 × ICPR 2447) and late (ICPA 2043 × ICPR 2671 and ICPA 2043 × ICPR 3467) maturing groups were identified. Segregation data for male fertility/sterility of BC1F1 and F2 populations showed involvement of two duplicate dominant genes in governing male-fertility. Two genetic maps based on ICPA 2039 × ICPR 2447 and ICPA 2043 × ICPR 2671 mapping populations with 82 and 117 SSR loci, respectively were developed. QTL analysis of the F2 mapping populations based on ICPA 2039 × ICPR 2447 and ICPA 2043 × ICPR 2671 detected six (four major and two minor) and four (one major and three minor) QTLs involved in the fertility restoration, respectively.
Page publiée le 22 septembre 2012, mise à jour le 1er février 2021