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Anna University (1997)

Comparison of molecular markers for developing pearl millet genetic linkage maps

Murali Krishna, I

Titre : Comparison of molecular markers for developing pearl millet genetic linkage maps.

Auteur : Murali Krishna, I

Etablissement de soutenance : Anna University.

Grade : Master of Science 1997

Résumé
Downy mildew (DM) disease caused by the fungus Sclerosprora graminicola (Sacc.) Schroet is the major yield reducer of pearl millet cropped in India and Africa. DM resistance is a complex, quantitatively - inherited trait. The available RFLP map of this crop developed at the Cambridge laboratory at Norwich, UK is useful for breeding for DM resistant pearl m llets by markerassisted selection and backcrossing. Further, the quantitative trait loci (QTLs) identified for resistance are also useful for searching for newer sources of resistance. The present work deals with identification of newer sources and markers for DM resistance in pearl millet. For this various types of molecular markers were compared. The marker types being compared includs Isozymes, RFLP, RAPD, and AFLP. Among the five accessions of pearl millet selected for the study three (7042 DMR P2 PI, iP 18298, and lP18292) are downy m ldew res stant and the other two accessions (8438 P5 P2, and 23 D2B ) are downy mildew suseptible. Out of the eight isozyme systems tried three (peroxidase, esterase, alcohol dehydrogenase) detected polymorphism between the test entries. RFLP analysis of these five accessions with the homologous genomic clones (PSM) in combination with 2 to 4 restriction enzymes (Dral, Hindlll, EcoRI, EcoRV) have revealed several polymorphic bands. RAPD assays with the same pearl millet accessions and 16 random primers showed that 50% of the RAPD primers identified one or more polymorphic bands. AFLP assay of two accessions 843 B PS P1 and 7042 DMR P2 PI revealed several bands in a 5% denaturing polyactylamide gel. All the seven primers tested showed polymorphism. AFLP seem to be technique of choice for further characterization of power of resistance and genetic linkage mapping.

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