Informations et ressources scientifiques
sur le développement des zones arides et semi-arides

Titre : Development of transgenic plants of groundnut (Arachis hypogaea L.) for induced resistance to groundnut rosette disease

Auteur : Daniel, S

Etablissement de soutenance : Jawaharlal Nehru Technological University

Grade : PhD thesis 2002

Résumé partiel
Impro ement ol’ disc ;lsc resistance in groundnlit (.lvo’,/li.\ I jp( ,ctrrv I..) h) hiorechnological approache* has heen limited due to the lock 01’ cflicielit protocols to regcncratc bhole plants through in vitro rcgc iclntion 111’:1d\cntitiou\ \11oot hudh li. rni transli rmcd tisrue .l’he prllnar ! i hjecti c ol’11iis \\OIL \\as to dc\clop cl’licicnt protocolr 11)r producing tra1i3genic grou id lut pl:1111s lilr induced rcrist,il cc to gruu idnut roscnc disease (GKI)). \rhich is cauacd by n comple\ of tlircc biru er that include groundnu[ rosette virus (GRV), groundnut rosette awstor \iru\ ((il<,t\’) r lid il i : tcllitc I(N.2 (ha[ IKNA1 4niongst Larious rscdling chplants. slioor rn rrpln gc icsi\ \+as i iduccd Ij.(llii llic pct olar cut end of leaflet esplnnts dcriwd lion1 I d-old in vitnl gro\rn secdliligs (11’ groundnut genotype IC’(iS-44, A colnhination or 13.3 pM I\ ;"-hc i/ ladcnitic (I3A) ;ind 5.3 thl u-nopIith : lene acetic acid (NAA) in tlic Murosliige a id Cioog’a liicdiu n (MSl \\ ;I\ III ILII I for itxlucirig ini lriple \Iiootr, I lic \lioot Ixid d l’fcrc it : t c i o urrccl in :I i c liir lashion fro ii tissuo of tile pctiolar cut ciid \\ tIiin 2 ucck\ 01’culturc ill ovcl. O ?’h c fthe ciilturcd crplants. I lie developnirnt of shoot buds into elongated \boats and tlicil. rooting uas achieved on a hormone-free MS medium ’I’he rooted shoots could he readill : ccliniati/cd in pol\ containing in vitro regenerated plantlets covered witli polythcnc hags, and mailitaincd in a glasshouse uith over 90% success ratc. In the glaahou\r plants exhibited normal growth and morphology. and upon maturity produced b ahle sccds. ’l’hc mc liod Tor regeneration of uhole plants developed in this study i\ widely applicable to dilf’erent genot)pes of Aruchi. ! hjpojiueu I..

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