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Accueil du site → Doctorat → Inde → 2002 → Development of transgenic plants of groundnut (Arachis hypogaea L.) for induced resistance to groundnut rosette disease

Jawaharlal Nehru Technological University (2002)

Development of transgenic plants of groundnut (Arachis hypogaea L.) for induced resistance to groundnut rosette disease

Daniel, S

Titre : Development of transgenic plants of groundnut (Arachis hypogaea L.) for induced resistance to groundnut rosette disease

Auteur : Daniel, S

Etablissement de soutenance : Jawaharlal Nehru Technological University

Grade : PhD thesis 2002

Résumé partiel
Impro ement ol’ disc ;lsc resistance in groundnlit (.lvo’,/li.\ I jp( ,ctrrv I..) h) hiorechnological approache* has heen limited due to the lock 01’ cflicielit protocols to regcncratc bhole plants through in vitro rcgc iclntion 111’:1d\cntitiou\ \11oot hudh li. rni transli rmcd tisrue .l’he prllnar ! i hjecti c ol’11iis \\OIL \\as to dc\clop cl’licicnt protocolr 11)r producing tra1i3genic grou id lut pl:1111s lilr induced rcrist,il cc to gruu idnut roscnc disease (GKI)). \rhich is cauacd by n comple\ of tlircc biru er that include groundnu[ rosette virus (GRV), groundnut rosette awstor \iru\ ((il<,t\’) r lid il i : tcllitc I(N.2 (ha[ IKNA1 4niongst Larious rscdling chplants. slioor rn rrpln gc icsi\ \+as i iduccd Ij.(llii llic pct olar cut end of leaflet esplnnts dcriwd lion1 I d-old in vitnl gro\rn secdliligs (11’ groundnut genotype IC’(iS-44, A colnhination or 13.3 pM I\ ;"-hc i/ ladcnitic (I3A) ;ind 5.3 thl u-nopIith : lene acetic acid (NAA) in tlic Murosliige a id Cioog’a liicdiu n (MSl \\ ;I\ III ILII I for itxlucirig ini lriple \Iiootr, I lic \lioot Ixid d l’fcrc it : t c i o urrccl in :I i c liir lashion fro ii tissuo of tile pctiolar cut ciid \\ tIiin 2 ucck\ 01’culturc ill ovcl. O ?’h c fthe ciilturcd crplants. I lie developnirnt of shoot buds into elongated \boats and tlicil. rooting uas achieved on a hormone-free MS medium ’I’he rooted shoots could he readill : ccliniati/cd in pol\ containing in vitro regenerated plantlets covered witli polythcnc hags, and mailitaincd in a glasshouse uith over 90% success ratc. In the glaahou\r plants exhibited normal growth and morphology. and upon maturity produced b ahle sccds. ’l’hc mc liod Tor regeneration of uhole plants developed in this study i\ widely applicable to dilf’erent genot)pes of Aruchi. ! hjpojiueu I..

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