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Accueil du site → Doctorat → Mexique → Estimación de estabilidad y evaluación agronómica y fisiológica de germoplasma de frijol común seleccionado por rendimiento de grano y daño por Macrophomina phaseolina

Universidad Autonoma de Nuevo Leon (2011)

Estimación de estabilidad y evaluación agronómica y fisiológica de germoplasma de frijol común seleccionado por rendimiento de grano y daño por Macrophomina phaseolina

García Olivares, Jesús Gerardo

Titre : Estimación de estabilidad y evaluación agronómica y fisiológica de germoplasma de frijol común seleccionado por rendimiento de grano y daño por Macrophomina phaseolina

Auteur : García Olivares, Jesús Gerardo

Université de soutenance : Universidad Autonoma de Nuevo Leon

Grade : DOCTOR EN CIENCIAS AGRÍCOLAS 2011

Résumé partiel
Charcoal rot [Macrophomina phaseolina (Tassi) Goid.] is an important disease of beans and other crops, particularly in northeastern México. The development of fungus resistant germplasm adapted to the conditions of the region, recurrent drought, is seen as a solution in the medium and long term. This work was conducted in two stages. In the first one, two methods of stability are compared analyzing the resistance to charcoal rot and grain yield [Eberhart and Russell (1966) and AMMI plus Biplot (Burgueño et al., 2001)] in 98 recombinant inbred lines (RELs) F8:10 and their parents (BAT-477 and Pinto UI-114). The experiments were planted in 10 x10 lattice design with four replications at four locations : Isla and Cotaxtla, Veracruz and Rio Bravo and Diaz Ordaz, Tamaulipas from 2007 to 2009. Two repetitions of each experiment were kept under irrigation and the other two were subjected to terminal drought after flowering. The second stage was to evaluate agronomic (field) and physiologically (greenhouses) of the five RELs (33, 62, 54 57 and 58) selected for high grain yield and lower severity of M. phaseolina in Veracruz and Tamaulipas along with the parents and three regional controls (Pinto Anzaldúas, Negro Michigan and Pinto San Juan). Two field experiments were carried out also with these ten genotypes in Rio Bravo and Diaz Ordaz in 2008 y 2009 using a randomized complete block design with four replications under irrigation. In the greenhouse (Marín, Nuevo León) were studied physiological variables (water relations, photosynthesis, stomata) and ’physiological quality’ in 2010. For the measurement of plant physiological variables were maintained soil moisture at field capacity until the beginning of pod formation and then subjected to drought (50% moisture in the soil to harvest). The treatments were arranged in a completely randomized design with three replicates with two plants per experimental unit. To evaluate the physiological quality were estimated numbers of stomata and under two temperatures (30 and 40 0C) of incubation. In this experiment, the germplasm was established under a randomized complete block design with split plot factorial arrangement and one plant per experimental unit.

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