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Université McGill (2006)

Antimicrobial and antioxidant activity of two desert truffles, Tirmania and Terfezia

Saleh A. Ali

Titre : Antimicrobial and antioxidant activity of two desert truffles, Tirmania and Terfezia

Auteur : Saleh A. Ali

Université de soutenance : Université McGill

Grade : Master of Science 2006

Résumé
Two species of desert truffles, Tirmania and Terfezia were collected from the Northern desert region in Saudi Arabia for antimicrobial and antioxidant activity testing. Both species were extracted with four types of extraction solutions, methanol, ethanol, ethyl acetate and water to test for antimicrobial activity and with three extraction solutions, methanol, ethanol and water for antioxidant activity. Using disc diffusion method, the extracts were subjected to twenty three different microorganisms to observe the antimicrobial activity by measuring clear zones. Methanol extract from Tirmania was the most effective, followed by those extracted with ethanol, water and ethyl acetate respectively. In Terfezia, ethanol extract was better than methanol extract in effectiveness. Ethyl acetate extracts were the least effective. The results indicate that truffles possess antimicrobial activity with broad spectrum effects against Gram positive, Gram negative, aerobic and anaerobic bacteria as well as Saccharomyces, while no effect was recorded with fungi. The results on antioxidant activity showed that truffles have very strong antioxidant property with 99.9% with ethanol extracts of Tirmania species and 95.5% with ethanol extract of Terfezia species using beta-carotene bleaching method and antioxidant property with 96.1% with ethanol extracts of Tirmania species and 95.3% with methanol extract of Terfezia species using DPPH free radical method

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Page publiée le 31 octobre 2012, mise à jour le 6 février 2018