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Accueil du site → Master → Chine → 2009 → Studied on Population Structure and Plant Pathogenic of Fusarium on Alfalfa Crown and Root in Arid Irrigation Area

Gansu Agricultural University (2009)

Studied on Population Structure and Plant Pathogenic of Fusarium on Alfalfa Crown and Root in Arid Irrigation Area

Zhao Hui

Titre : Studied on Population Structure and Plant Pathogenic of Fusarium on Alfalfa Crown and Root in Arid Irrigation Area

Auteur : Zhao Hui

Grade : Master’s Theses 2009

Université : Gansu Agricultural University

, the alfalfa root root collar Fusarium population structure alfalfa growth period, the different seasons, from Gansu Province, Jingtai County Irrigation District incidence alfalfa fields to take incidence sample, using conventional indoor organizational separation technology, the initial separation of Fusarium. Single spore isolates isolated from Fusarium in WA, go to the PSA and PDA traits observed, measured growth rate, the CLA-induced sporulation. 257 Fusarium isolates identified are nine kinds, including F. solani (F. solani) is the most dominant species isolated from 93 isolation frequency of 36.41% ; followed by half-naked Fusarium (F. semitectium ), the the sickle of Fusarium (F.fusarioides) and Fusarium (F. oxysporium), isolation frequency, respectively 25.27%, 10.20% and 8.89%, respectively. Fusarium Species group change the Alfalfa different growth period, the population size of the root and root collar Fusarium different, but each season of the dominant species are F. solani (F. solani). Sampled in early April regreening this isolated 67 Fusarium ; to 52 June Growing Season isolated ; August 99 flowering stage separation ; in October received mow isolated 39. Determination of pathogenic Fusarium Indoor plate inoculation method, Determination of pathogenic varieties Debao, there are differences between the different species of Fusarium pathogenicity, the same species of Fusarium, between different strains of pathogenic resistance are also differences. F. solani, half-naked Fusarium, Fusarium, sharp top Fusarium, corners Fusarium strains pathogenic and control compared can reach a significant level, and sickle Fusarium 15 # #, oats, equisetum # 1, # 2, non-spore group 13 # 14 # 15 # 16 # pathogenicity and control showed no significant differences, initially identified as weakly pathogenic strains. Sickle Fusarium (F.fusarioides), corners Fusarium (F.camptoceras) can cause alfalfa roots and root rot is the first report on the alfalfa. 4, weakly pathogenic Fusarium pathogenicity of other varieties of alfalfa select the alfalfa varieties \Equisetum # 1, # 2, non-spore group 13 # 14 # 15 # 16 #. Xinjiang big leaf, Algonquin, Golden Queen, Gannon, alfalfa king, madrigal, Delhi Rand, Hunter River, An Guli, Shaanxi moment alfalfa 10 varieties, using the plate method for the determination of the different varieties of alfalfa pathogenicity. Test on the 10 species found eight weak pathogenic strains pathogenic differences, but the whole showed weak pathogenic non-spore group 14 # and 14 # sickle basic non-pathogenic strains after each treatment ; 5, attenuated strains of Fusarium growth of alfalfa seedlings 8 weakly pathogenic sickle strains of dealing with 10 different varieties of alfalfa seedlings radicle length shorter than the control, and the difference is significant. 13 # strains of non-spore can cause an increase in fresh weight of the different varieties of alfalfa seedlings, and control differences significant ; no significant increase in the other strains. 6, non-pathogenic Fusarium control rot of alfalfa seedlings inoculated with non-pathogenic Fusarium strains pathogenic strains pathogenic to dampen demand, be strong, but belong to the same kind of weak pathogenic sickle sickle spore virulent sickle Fusarium no inhibition and the first inoculation weak (non) pathogenic strains after inoculation strong pathogenic strains, the pathogenic role than inoculation weak (non) pathogenic strains and the strong pathogenic strains should be obvious.

Mots clés : Alfalfa Fusarium Populations Pathogenicity

Présentation (Dissertation topics )

Page publiée le 1er mai 2013, mise à jour le 11 mars 2018