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Northwest University of Science and Technology (2008)

Study on Actinomycete Resources from Desert and Saline-Alkali Soil in Xinjiang Province

赵卉琳; Zhao Hui Lin

Titre : Study on Actinomycete Resources from Desert and Saline-Alkali Soil in Xinjiang Province

Auteur : 赵卉琳; Zhao Hui Lin

Grade : Master’s Theses 2008

Université : Northwest University of Science and Technology

Résumé partiel
Xinjiang located in eurasia hinterland, the territory is very vast, special and diversiform ecological environment gestates special and diversiform microbial resources, so it has great researching and developing value. Study on actinomycete resources from desert and saline-alkali soil in part of region of Xinjiang, which has important significance for law of actinomycete biodiversity in this kind of habitat and promoting exploition of extreme microbial resources, meanwhile, which provide theoretical basis for exploition of extreme actinomycete resources.25 soil samples from desert and saline-alkali soil in Turpan、Kuala、Aksu、Kashgar、Ili and Khotan ect.of Xinjiang were collected, by means of testing soil basic properties analysis the law of ecological distribution of actinomycete, 632 strains of actinomycetes were obtained. Taking common plant pathogens and animal pathogens as organism, which damage seriously in agricultural production and animal husbandry culture, we screened excellent antagonistic actinomycetes, and used polyphasic taxonomy technique to identify parts of strains of actinomycetes which had special morphology and excellent antagonistic effect, meanwhile, preliminary discussed isolation method of actinomycete.The main conclusions were drawed by our research:1. Ecological background of experimental soil and composition of actinomycetes populationsExperimental soil organic matter average contents were lower than middle level, solonetz soils > gravel solonetz soils > sand saline soils > sand soils and average contents were 6.54 21.83、3.75 26.23、2.42 11.31、1.75 8.36 g·kg-1 respectively ; available nutrients contents varied slightly, available nitrogen、available phosphorus、available potassium average contents were 26.37 78.30 mg·kg-1、7.89 13.30 mg·kg-1、156.11 431.37 mg·kg-1 respectively ; Soil pH value varied around 8 ; Soil total salt of solonetz soils、gravel solonetz soils、sand saline soils and sand soils average contents were 0.46 2.64、0.28 0.65、2.96 80.12、0.20 1.46 g·kg-1 respectively, and average contents were highest in sand saline soils.605 strains of actinomycetes were obtained, and 533 strains were Streptomyces, Streptomyces accounted for 88.1 % of total actinomycetes, Streptomyces could be classified into 12 kinds and Cinerogriseus was the dominant group. There were 72 strains of other genera, and accounted for 11.9 % of total actinomycetes, they could be classified into Nocardia、Micromonospora and Indeterminate genera, Nocardia was the dominant genera.The statistical analysis showed that the positively significant correlation were found out between the contents of soil available phosphorus and the quantities and species of soil actinomycetes, the contents of soil available nitrogenous were correlated with the quantities of soil actinomycetes positively, the contents of soil total salt were correlated with the the quantities and species of soil actinomycetes negatively, soil organic matter and available phosphorus had most effect on the quantities and the species of soil actinomycetes.2. Discussion of isolation method of actinomyceteThe results showed that the quantities and species of actinomycetes were the most when thermal treatment (100℃×1 h) ; There were the most of the quantities of actinomycetes after the treatment of 6 % peptone solution, but species were the least, the quantities of actinomycetes were in second place after 0.05 % SDS solution, while there were many species ;

Mots clés : Xinjiang; Desert and saline-alkali environment; Ecology of actinomycetes; Antagonistic actinomycetes; Isolation method; Identification; Polyphasic taxonomy;

Présentation (CNKI)

Page publiée le 2 mai 2013, mise à jour le 28 novembre 2017