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Shandong University (2008)

Comparative Study on Roteomics between Salt and Drought Stress of an Introgression Line of Wheat

Peng Zhen Ying

Titre : Comparative Study on Roteomics between Salt and Drought Stress of an Introgression Line of Wheat

Auteur : Peng Zhen Ying

Grade : Doctoral Dissertation 2008

Université : Shandong University

At the present time, environment is becoming worse and worse, which has harmful impact on agricultural productivity around the world. In all, the useable lands for cultivation are less than 10%, the great mass of lands are in drought, salinity, quagmire and cold. Therefore, it is an important task to dissect the mechanisms of salt and drought adaptive plants with the aim to cultivate new salt and drought tolerant varieties.In our group, a new wheat variety, Shanrong No. 3 (SR3), with high salt/drought tolerance and high yield was successfully bred via asymmetric somatic hybridization between wheat (Triticum aestivum L. cv. Jinan177 (JN177) and Thinopyrum ponticum. This novel cultivar is an introgression line containing chromatin of Thinopyrum ponticum. In this study, a proteomic study was conducted to determine the difference in young root and leaf proteomes of SR3 and its somatic hybrid parent JN177. Differences in proteomics response of the hybrid and the parent between drought and salt stress were analysed. RT-PCR assay was used to analyse the transcription patterns of 25 selected differentially expressed proteins. Taken together, responsive characteristics of SR3 and JN177 under salt/drought stress were systematically investigated at transcriptional and translational levels, and the mechanism of salt/drought resistance is discussed:1. In total, 93 differentially expressed proteins were obtained in roots of JN177 and SR3 seedlings under drought and salt stress. Of them, eighty-eight were identified by MALDI TOF-TOF MS. First, root proteome of SR3 was markedly different from that of JN177. Results showed that thirteen spots were special to SR3 and 21 spots were particular to JN177. Second, significant difference was there in expression patterns of salt/drought-induced proteins between SR3 and JN177. Among the 20 salt/drought-induced proteins, three were specific to SR3 and five were specific to JN177, the others were induced in both of them. Third, there were distinguishing differences in expression patterns between salt- and drought-responsive proteins. Compared with drought stress, Up-regulated and down-regulated proteins under salt stress were increased by 19.2% and 11.7% respectively. This suggested that salt stress could bring harder and broader stress responsive processes and changed the expression patterns of more proteins than drought stress. The other proteins such as quinone reductase (root 90) and eukaryotic translation initiation factor 5A3 (root 74) were down-regulated under drought stress but inhibited under salt stress in roots of JN177 suggested that there were some difference in signal transduction pathways between salt and drought stress. Fourth, there were variety-differences in expression patterns of salt/drought stress responsive proteins between SR3 and JN177, and it was shown that expression patterns of the same protein were different between JN177 and SR3. For instance, nucleotide-excision repair protein (root 21) was down-expressed in JN177 but unchanged in SR3 under salt/drought stress ; Aldolase (root 8) was unchanged in JN177 but up-regulated in SR3 under drought stress ; eukaryotic translation initiation factor 5A3 (root 74) was inhibited in JN177 but down-regulated in SR3. It was the somatic hybridization that changed the expression patterns of stress responsive proteins.2. Sixty-five differentially expressed proteins were found in leaves of JN177 and SR3 seedlings under salt and drought stress, 63 ones were identified by MALDI TOF-TOF MS. Comparative analysis showed that the state was similar to roots. For example, there were also remarkable differences in expression patterns between salt-and drought-responsive proteins in leaves : chlorophyll a/b-binding apoprotein CP24 precursor (leaf 1) was increased by 12.7 times under drought stress in SR3 whereas increased by 6.1 times under salt stress ;

Mots clés : Proteomics Two dimensional gel electrophoresis MALTI-TOF/TOF-TOF MS RT-PCR Salt tolerance Drought resistance Somatic hybridization Wheat

Présentation (Dissertationtopic)

Page publiée le 3 mai 2013, mise à jour le 9 janvier 2018