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Accueil du site → Doctorat → Chine → 2007 → Reproduction Characteristics Of The Typical Desert Moss And Mechanisms Of Physiological And Biochemical Tolerance To Environmental Dehydration And Thermostress

Shanghai Jiaotong University (2007)

Reproduction Characteristics Of The Typical Desert Moss And Mechanisms Of Physiological And Biochemical Tolerance To Environmental Dehydration And Thermostress

许书军; Xu S J

Titre : Reproduction Characteristics Of The Typical Desert Moss And Mechanisms Of Physiological And Biochemical Tolerance To Environmental Dehydration And Thermostress

Auteur : 许书军; Xu S J

Université de soutenance : Shanghai Jiaotong University

Grade : Doctor (Dissertation) 2007

Résumé
Through investigation the desert moss spinosa wall moss tissue culture influencing factors, to explore the possibility of rapid propagation of the desert moss and its biological crusts indoor, large-scale cultivation the desert moss as well as the biological crusts provide basic research data ; through the survey moisture, chlorophyll fluorescence, conductivity, pigment content, ultrastructure and regeneration potential of traditional indicators, evaluation thorn leaf wall moss different spin speed and different temperatures, the response in morphology, physiology and reproductive biology, desert region moss biological Results reasonable skin protection and repair of guiding significance ; scanning electron microscopy (SEM), the micro infrared (Micro-FTIR) and single reflection (ATR) technology, the mark-on-demand paramagnetic resonance (EPR Spin Label), in conjunction temperament (GC / MS ), used in conjunction liquid chromatography (LC / MS), ion chromatography (IC) thermal power measurement tools (TGA and DSC), time-of-flight mass spectrometry (MALDI-TOF) and other new technologies used in the moss patience research, and explore the thorn leaf wall moss chemical composition in the surface structure and waxy chemical, cell gel state - liquid crystalline state - glassy mutual changes in cell membrane permeability and cytoplasmic molecular mobility and membrane interface structure on the different spin speed and temperature response, moss biology research breakthroughs, more research is relatively low and opened up a new way to form a smaller organisms. At the same time, the use of two-dimensional electrophoresis, compared dehydration - rehydration spinosa wall the moss protein expression difference, screening spinosa wall moss resistance to dry functional proteins, for the desert moss dry heat resistance gene Isolation and functional genes in crop improvement the application of the foundation. First of all, according to the tissue culture method investigated several common factors such as disinfection methods, media, hormones, culture temperature, explant, humidity and light intensity and time of regeneration of the spinosa wall moss. Ultimately determine the the spinosa wall moss rapid propagation route : explants from leaves → temperature 25/15 ° C → nutrition source Knop → RH 65-80% → the photoperiod 16H → light intensity 85-120 PPFD → culture period 2 months material without disinfection ; grasp the spinosa wall moss growth influencing factors, try to build moss crust induced Protonema : isolated green leaves at 20/10 ° C, and then transferred to river sand, get the original moss crust , indicating the presence of moss crust artificial cultivation possible. In order to understand the desert moss the desert common moisture and temperature stress resistance mechanisms, dehydration and high temperature and joint processing spinosa wall moss response in terms of morphology, physiology and reproductive characteristics. Form spinosa wall moss has obvious ecological advantages : (1) double cell wall and fat granule-rich, in which the aging organization than the new organization more patience advantages ; different developmental stages of leaf cell chloroplasts round (2) rapid dehydration treatment, there was no cell wall and membrane damage ; ③ 45 ° C heat treatment is caused by loose chloroplast structure only ; ④ 45 ° C dry heat treatment to produce a large number of bubbles and structural distortions caused by chloroplast part of the cell membrane damage, showed that 45 ° C under dehydration and temperature on the cell membrane exist synergies ; ⑤ rapid dehydration, 45 ° C heat treatment, 45 ° C dry heat treatment after the chloroplasts of leaf cells after rehydration able to restore normal morphology. (3) Some Stress chlorophyll fluorescence physical spinosa wall moss has obvious anti-dry heat response : (1) several stress caused by a large number of cytosolic leakage ; (2) pigment and soluble sugar content only hot and dry heat of both severe response ; decreased activity, dehydration and dry heat treatment leaves light system to quickly return to normal levels, high temperature treatment (≥ 50 ° C) loss of fluorescence activity, indicating that the lethal effects the extreme high Wen Duiguang of the system ; ④ high temperature reduce gill leaf wall moss regenerative capacity , but hot and dry joint stress (lt ; 60 ° C) no ability to regenerate a significant impact ;

Mots clés : Tortula desertorum Broth., ecological reproduction, physiological response, structural chemistry, proteomics, environmental stresses, desiccation tolerance and thermotolerance

Présentation (DissertationTopic)

Page publiée le 8 mai 2013, mise à jour le 24 avril 2018