Informations et ressources scientifiques
sur le développement des zones arides et semi-arides

Accueil du site → Master → Canada → Functional Genomic Investigation of Aromatic Aminotransferases Involved in Ephedrine Alkaloid Biosynthesis in Ephedra Sinica (Stapf).

University of Western Ontario (2012)

Functional Genomic Investigation of Aromatic Aminotransferases Involved in Ephedrine Alkaloid Biosynthesis in Ephedra Sinica (Stapf).

Kilpatrick, Korey G

Titre : Functional Genomic Investigation of Aromatic Aminotransferases Involved in Ephedrine Alkaloid Biosynthesis in Ephedra Sinica (Stapf).

Auteur : Kilpatrick, Korey G

Université de soutenance : University of Western Ontario

Grade : Master of Science 2012

Résumé
Ephedra sinica (Ephedraceae) is a broom-like shrub cultivated in arid regions of China, Korea and Japan. This medicinal plant accumulates large amounts of the ephedrine alkaloids, including (S)-cathinone, (1R,2S)-norephedrine, (1R,2S)-ephedrine and (1S,2S)-pseudoephedrine in its aerial tissues. These analogues of amphetamine mimic adrenaline and stimulate the sympathetic nervous system. While much is known about their pharmacological properties, the biological mechanisms by which they are synthesized remains largely unknown. A functional genomics platform was established in order to investigate alkaloid biosynthesis. RNA was extracted from Ephedra sinica stems and sequenced by Illumina HiSeq2000 next-generation sequencing. Candidate biosynthetic enzymes were obtained from this EST collection based on similarity to characterized enzymes with similar functions. This portion of the collaborative study is focused on identifying aminotransferase enzymes involved in alkaloid biosynthesis. Two functional aromatic aminotransferase enzymes, possibly involved in ephedrine biosynthesis have been identified. One exhibiting much greater enzymatic activity was chosen for characterization

Mots clés : Aminotransferase ; Ephedra sinica ; ephedrine alkaloids ; functional genomics ; medicinal plants ; next-generation sequencing ; Genetics and Genomics ; Molecular Biology

Présentation

Version intégrale (3,4 Mb)

Page publiée le 20 juin 2013, mise à jour le 1er février 2018