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Jawaharlal Nehru Technological University (2012)

Linkage mapping and QTL analysis of drought related traits in Groundnut (Arachis hypogaea L.)

Gautami, B

Titre : Linkage mapping and QTL analysis of drought related traits in Groundnut (Arachis hypogaea L.)

Auteur : Gautami, B

Université de soutenance : Jawaharlal Nehru Technological University

Grade : Doctor of Philosophy (PhD) in Biotechnology 2012

Résumé partiel
Groundnut (Arachis hypogaea L.), commonly called peanut, is one of the most important oilseed crops in the smallholder-farming sectors of the semi-arid tropical regions of the world where drought is the major production constraint. Until recently, the low level of molecular diversity in the cultivated groundnut genome and the scarcity of co-dominant DNA-based molecular markers were critical constraints in using modern genomics in groundnut improvement. To increase the number of molecular markers for groundnut, 23 novel simple sequence repeat (SSR or micro-satellite) markers were isolated from a SSR-enriched genomic library. These new markers, along with 3215 already available markers from different sources were tested for detecting polymorphism among parental genotypes of the two recombinant inbred line (RIL) mapping populations (ICGS 76 × CSMG 84-1 and ICGS 44 × ICGS 76) to understand the genetic basis and identification of QTLs for drought related traits. As a result, two new genetic linkage maps were developed with 119 (2208 cM) and 82 (831 cM) marker loci. In addition, a consensus map consisting of 293 SSR loci located across 20 linkage groups and spanning a map distance of 2841 cM was constructed using the two new genetic maps (from the present study) and the reference map TAG 24 × ICGV 86031....

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