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Accueil du site → Doctorat → Chine → 2013 → Effects of High Temperature, Drought Stress and Their Combination on Glutenin Macropolymer Patricle Distribution, Starch Granule Distribution and Quality Traits in Wheat

Henan Agricultural University (2013)

Effects of High Temperature, Drought Stress and Their Combination on Glutenin Macropolymer Patricle Distribution, Starch Granule Distribution and Quality Traits in Wheat

Lu Hong Fang

Titre : Effects of High Temperature, Drought Stress and Their Combination on Glutenin Macropolymer Patricle Distribution, Starch Granule Distribution and Quality Traits in Wheat

Auteur : Lu Hong Fang

Grade : Doctoral Dissertation 2013

Université : Henan Agricultural University

Résumé partiel
To understand effects of post-anthesis high temperature (HT) and drought stress(DS) on wheat grain quality and yield, the present studies, two different wheatcultivars with strong-and mid-gluten (Zhengmai366and Yunong949) used,combined pot culture with climate-controlled greenhouse simulating high temperature,were carried out to characterize effects of HT, DS and their combination on the starchgranule distribution, glutenin macropolymer particle size distribution and keyenzymes relating to N metabolism in wheat grains, and analyse the variation in starchcomponents, characteristics and protein concentration, components as well as thegrain yield. This aimed to investigate the internal mechanisms and scope that stressinfluenced on the starch and protein quality in wheat grains, and make a theoryevidence for the wheat production of high yield and high quality resisting the stress.The main results are as follows:1. The present results showed that the number distribution showed one-peakcurve, while the volume and surface area distribution all showed two-peak curve indifferent treatments. High temperature, drought stress and the combination of thesestressors during grain filling stage mainly influenced the starch volume and surfacearea distribution, but had less effect on the number distribution. High temperature,drought stress and their combination all led to a significant reduction in the volumeproportion of A-type starch granules (>9.9μm) and the surface area proportion ofgranules <2.8μm, and a significant increase in the volume proportion of granules2.8-5.8μm and5.8-9.9μm in Zhengmai366with strong-gluten. Whereas, in Yunong949with mid-gluten, HT, DS and their combination all led to a significant increase inthe volume and surface area proportion of A-type starch granules, yet a significantdecrease in the volume proportion of granules2.8-5.8μm and5.8-9.9μm. The starchgranule distribution of Zhengmai366with strong-glutenin was more sensitive tostress than Yunong949. The degree of different stress influencing the starch volume distribution showed that the combination of HT and DS at early stage of grain fillingwas greater than other stresses, following were HT applied at early stage, DS, HTapplied at mid-stage of grain filling, and the last was the combination of HT and DSat mid-stage. The starch granules were notably distorted and volume reduced by HTand DS in scanning electron microscope.2. High temperature, drought stress and its combination significantly affected thestarch concentration and its components as well as the starch characteristics in wheatgrains. The concentrations of amylose, amylopectin and total starch all decreasedunder stress treatments, yet the amylose was less sensitive to stress than amylopectinand total starch, which led to the increase in ratio of amylose to amylopectin. Thepasting characteristics of peak viscosity, hold through, final viscosity, breakdown andsetback all significantly increased in Zhengmai366and decreased in Yunong949under DS, while, that significantly declined in Zhengmai366and Yunong949underHT and the combination of HT and DS. The swelling power declined in Zhengmai366and increased in Yunong949under DS, whereas, that decreased in the two wheatcultivars under HT and the combination of HT and DS

Mots clés : winter wheat high temperature drought stress starch pastingcharacteristics protein enzymes activity glutenin macropolymer (GMP)

Présentation (Dissertation topics)

Page publiée le 6 avril 2014, mise à jour le 10 janvier 2018