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Istituto Agronomico Mediterraneo di Bari (IAMB) 2004

Ecological and biological investigations on Aureobasidium pullulans, Penicillium spp. and their interaction on citrus fruits

Oubaki, Lahoucine

Titre : Ecological and biological investigations on Aureobasidium pullulans, Penicillium spp. and their interaction on citrus fruits

Auteur : Oubaki, Lahoucine

Etablissement de soutenance : Istituto Agronomico Mediterraneo di Bari (IAMB) - Centre international de hautes études agronomiques méditerranéennes (CIHEAM)

Diplôme : Master of science : Integrated pest management of Mediterranean fruit crops 2004

Résumé
Oranges were collected from organic and conventional farms located in Basilicata (ltaly) to study the effect of the farming system on epiphytic microbial population on citrus fruit surface. The populations of filamentous fungi, Aurebasidium pullulans, and yeasts were higher in organic orchards without any treatment in comparison with conventional ones. Whereas, no significant differences in terms of this population were observed between conventional and organic orchards treated with copper. The effectiveness of the antagonist Aureobasidium pullulans, strain L47, in controlling green and blue moulds, was evaluated when applied in preharvest, both in preharvest and postharvest, or only on postharvest. Treated fruits were kept at 20.C for 30 days. When applied as postharvest treatment, the antagonist was more effective in controlling green and blue moulds than preharvest one. Techniques based on random amplified polymorphic DNA (RAPD) and arbitrarily primed polymerase chain reaction (AP-PCR) were used to determine the genetic variability among populations of Penicillium spp. collected from ltaly and Morocco, both in orchards and packinghouses. With the tested primers, cluster analysis using UPGMA showed a higher genetic variability in P. italicum isolates than P. digitatum. The pathogen P. ulaiense, was not found among isolates collected from packinghouses and fields in Morocco.

Mots-clés : CITRUS PREHARVEST TREATMENT POSTHARVEST TREATMENT POSTHARVEST DECAY BIOLOGICAL CONTROL AUREOBASIDIUM PULLULANS PCR RAPD

Présentation (IAMB Library)

Page publiée le 14 juin 2014, mise à jour le 11 septembre 2018