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Instituto Agronómico Mediterráneo de Zaragoza (IAMZ) 2006

Estudio de determinantes de patogeneicidad y resistencia derivada del patógeno en cítricos transgénicos como estrategia de protección frente al Virus de la Tristeza de los Cítricos (CTV)

SOLER CALVO Nuria

Titre : Estudio de determinantes de patogeneicidad y resistencia derivada del patógeno en cítricos transgénicos como estrategia de protección frente al Virus de la Tristeza de los Cítricos (CTV)

Auteur : SOLER CALVO Nuria

Etablissement de soutenance : Instituto Agronómico Mediterráneo de Zaragoza (IAMZ) - Centre international de hautes études agronomiques méditerranéennes (CIHEAM)

Diplôme : Master of Plant Breeding / Amélioration Génétique Végétale / Mejora Genetica Vegetal (2006)

Résumé
Citrus is the most important fruit tree crop in the world. Spain is the fifth worldwide producer and the first exporting country of fresh fruit according to data from FAO (2006). Citrus are subjected to abiotic and biotic stresses that limit their culture. Citrus tristeza virus (CTV) is the casual agent of the most important viral disease of Citrus, an it has supposed the loss of more than 80 million trees in the world. Nowadays, the tristeza disease is not a problem for the Spanish citrus industry. However, we depend almost exclusively on citrange rootstocks, and this makes citrus crop very vulnerable to the hypothetical appearance of aggressive mutants of the virus or of new pathogens that could affect citrange. In addition, the excellent sour orange rootstock cannot be used in our citrus culture. The main objective of this work is to obtain pathogen- derived resistance in transgenic citrus as a strategy of protection against CTV. The work has been divided in three chapters. First, a genetic transformation vector has been constructed with an expression cassette derived from the p18 gene from CTV. The use of p18 gene seems to be a good alternative for obtaining resistance against CTV, because it seems to be involved in cell-to-cell movement of the virus. Then, transgenic plants of Mexican lime (Citrus aurantifolia (Christm.) Swing.) have been generated which over-express different mutated versions of p23 from CTV.

pPésentation (AGRIS-FAO)

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Page publiée le 21 juin 2014, mise à jour le 10 juillet 2018