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Chinese Academy of Forestry (2010)

Rare desert shrub Helianthemum Reproductive Ecology and AFLP analysis of genetic diversity

Gao Zuo Zuo

Titre : Rare desert shrub Helianthemum Reproductive Ecology and AFLP analysis of genetic diversity

Auteur : Gao Zuo Zuo

Grade : Master’s Theses 2010

Université : Chinese Academy of Forestry

Résumé partiel
This nature reserve in the western Ordos core area and edge areas, through fixed sample, the sample strain observation and indoor and outdoor experimental analysis methods, the use of reproductive ecology, population ecology, reproductive biology and conservation biology and other subjects principles and research methods to rare species of desert regions Helianthemum (Helianthemum soongoricum Schrenk) as the research object, the individual, population, molecular three levels, the population of the entire reproductive process reproductive characteristics, reproductive law, reproductive and environmental relationships and reproductive adaptation strategies for a comprehensive system. Fixed sample selected in this study, were fixed, fixed lines, regular long-term observation. Indoors on Helianthemum pollen germination and pollen - ovule ratio (P / O) for experimental observations, outdoors through field of quantitative measurement, laboratory testing and indoor mathematical analysis and other means, in exploring Helianthemum population reproductive phenology law distribution based on the study of Helianthemum breeding system and reproductive and sexual reproductive process characteristics, measurement of Helianthemum population age structure and fertility, prepared Helianthemum Reproductive Value table ; studied Helianthemum populations in time and space Reproductive Allocation. Use of AFLP study does not similarly Helianthemum genetic diversity among individuals. Endangered Helianthemum brief analysis mechanism. The results show that : ⑴ Helianthemum populations are ongoing throughout the growing season flowering and fruiting, and precipitation closely related to rainfall and more in May and August flowering rate was significantly higher than in other months ; Helianthemum single flower for a day ; different times during the growing season, half-day flower characteristics with the Ministry of differences, in May and August two flowering, floral morphology largest value ; floral morphology at different slope positions were no significant differences. ⑵ by the pollen, ovule ratio of the number (P / O), hybridization index (OCI) and bagging assay, Helianthemum outcrossing breeding system, some self-compatible, you need pollinators, there is no apomixis, performance out on insect pollination adaptability. ⑶ Helianthemum populations in each of the growing season to produce a lot of flowers and buds, but the mature seed rarely, abortion rate is extremely high. At different times of the current theory of reproductive reproductive value and the actual value has a large gap between the current in the two breeding season when compared to other large value ; different slope positions in the current reproductive value and the seed rate had significant differences, in general is the slope gt ; slope in gt ; slope ; ⑷ Helianthemum fertility and reproductive value of the overall trends are divided as to ground diameter diameter class first and then decreases. Middle and lower slope slope spent half a day when the ground diameter 18 21mm fecundity maximum slope portion in the ground when the fertility diameter 15 18mm Max. Slope portion Helianthemum individual reproductive Max, followed by the lower slope, and finally the middle slope. Residual reproductive value (RRV) at different slope positions in descending order of population showed a trend ; cumulative residual reproductive value (SRRV) with diameter class increases. Total reproductive value (TRV) in the slope portion and the lower slope first increased and then decreased in the middle slope instability. Reproductive investment strategy (ORE) Ministry highest slope, sloping lower minimum ; ⑸ population reproductive allocation in various reproductive stages are quite different. But in different reproductive period, reproductive allocation populations Helianthemum reproductive period changes are presented as high - low - high - low trends ; 0-15mm in diameter class range, reproductive allocation increases as the diameter class increases, the different slope positions reproductive allocation order : upper slopes gt ; Singapore Central gt ; sloping bottom

Mots clés : Helianthemum Reproductive Ecology Breeding system Reproductive efficiency Reproductive value table Reproductive allocation AFLP Polymorphism Genetic similarity coefficient

Présentation (Dissertation topics)

Page publiée le 18 octobre 2014, mise à jour le 2 avril 2018