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Universidade Federal de Lavras (2006)

Avaliação do uso de agentes osmóticos e microssatélites na seleção de genótipos de trigo tolerantes à seca

Girotto, Larissa

Titre : Avaliação do uso de agentes osmóticos e microssatélites na seleção de genótipos de trigo tolerantes à seca

Evaluation os osmotic substances and microsatellits on selection of wheat genotypes tolerant to drought

Auteur : Girotto, Larissa

Université de soutenance : Universidade Federal de Lavras

Grade : Mestrado em Fisiologia Vegetal 2006

Résumé
The present paper aimed to evaluate the use of osmotic substances and microsatellits on selection of wheat genotypes tolerant to drought. Samples from eight genotypes werw evaluated by length of aereal part and main root in plantlets and immature embryos after water deficit. For this purpose there here used three induces of water loss in cell : polyetyleneglicol 6000, maltose, mannitol and water as control. In order to acess the genetic variability the from 93 cultivars / lineages were extracted and analyzed using a set of 42 microsatellits markers, distributed in different chromosome to acess the genetic similarity of the material and also polymorphism. The results obtained with relative length of aereal part and main root in plantlet on water deficit showed that the chemicals used, PEG 6000 e mannitol, caused stress in cultivars tested, being significative the interaction between these agents and the cultivar. PEG 6000 caused higher negative effects than mannitol. Considering the relative length of aereal part and main root in immature embryos submitted to water deficit, it was verified among the stress agents used, the mannitol and maltose were more toxic to the plant tissue studied and the PEG 6000 the osmoticum agent more adequate. In the experiment related to genetic variability in wheat genotypes tolerant to drought, the similarity mean coefficient for 93 cultivars / lineages was 0,691. the lineage with more close genetically were PF 81189 and PF 81191 (0,95), developed by Embrapa Wheat, and the genotypes more distant genetically were IPF 77781, IPF 77783 and IPF 78080 (0,50). There were verified a high polymorphism level among the cultivars in the Active Germoplasm Bank, mainly Aliança and BRS 207. The results of this research, trying to search early the differential tolerance to water deficit in plantlets and immature embryos of wheat, involving different osmotic substances and microsatellits, showed divergence with the field observations, given by Active Germoplasm Bank of the Embrapa Wheat.

Mots clés : Trigo Agentes osmóticos Tolerância Seca Wheat Osmotic substances Tolerant Drought

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