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Brigham Young University (2012)

Transcriptome Analysis of Drought Induced Stress in Chenopodium quinoa

Raney Joshua A.

Titre : Transcriptome Analysis of Drought Induced Stress in Chenopodium quinoa

Auteur : Raney Joshua A.

Université de soutenance : Brigham Young University

Grade : Master of Science (MS) 2012

Résumé
RNA-seq transcriptome analysis of Chenopodium quinoa at different water treatment levels was conducted in a greenhouse study using four water treatments (field capacity to drought) on a valley ecotype quinoa (variety Ingapirca) and an Altiplano Salares ecotype quinoa (variety Ollague). Physiological results support the earlier findings that the Salares ecotypes display greater tolerance to drought-like stress conditions than the valley ecotypes (as determined by growth rate, photosynthetic rate, stomatal conductance, and stem water potential). cDNA libraries from root tissue sample for each treatment x variety combination were sequenced using Illumina Hi-Seq technology in an RNA-seq experiment. De novo assembly of the transcriptome generated 20,337 unique transcripts. Gene expression analysis of the RNA-seq data identified 462 putative gene products that showed differential expression based on treatment and 27 putative gene products differential expressed based on variety x treatment, including significant expression differences in root tissue in response to increasing water stress. BLAST searches and gene ontology analysis show an overlap with drought tolerance stress and other abiotic stress mechanisms.

Mots clés : Chenopodium quinoa, drought, Illumina sequencing, RNA-seq, transcriptome assembly

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Page publiée le 13 février 2015, mise à jour le 9 novembre 2018