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Accueil du site → Projets de développement → Projets de recherche pour le Développement → 2010 → MOLECULAR, PHYSIOLOGICAL, AND AGRONOMIC CHARACTERIZATION OF SALT TOLERANT ALFALFA GERMPLASM

United States Department of Agriculture (USDA) 2010

MOLECULAR, PHYSIOLOGICAL, AND AGRONOMIC CHARACTERIZATION OF SALT TOLERANT ALFALFA GERMPLASM

United States Department of Agriculture (USDA) Research, Education & Economics Information System (REEIS)

Titre : MOLECULAR, PHYSIOLOGICAL, AND AGRONOMIC CHARACTERIZATION OF SALT TOLERANT ALFALFA GERMPLASM

Identification : UTAR-2009-02156

Pays : Etats Unis

Durée : Mar 1, 2010 à Feb 28, 2015

Domaine : Plant Biological Efficiency and Abiotic Stresses Affecting Plants ; Plant Genome, Genetics, and Genetic Mechanisms ; Basic Plant Biology ; ALFALFA

Partenaire : AGRICULTURAL RESEARCH SERVICE LOGAN,UT

Objectif
The ultimate goal of this research is to use a comprehensive approach to efficiently identify, characterize and develop alfalfa (Medicago sativa L.) germplasm with improved salinity tolerance. The objective of this current project is to evaluate the agronomic performance of four greenhouse-selected salt tolerant alfalfa populations and to understand the molecular genetics and physiological mechanisms underlying the salt tolerance. Alfalfa is an important forage crop in the US. Improved cultivars with high production on saline soils will benefit many producers and land managers. The specific objectives of the project are : Objective 1. Quantify the agronomic performance, forage quality and morphological characteristics of four experimental salt-tolerant alfalfa populations on saline and non-saline irrigated soils. It is anticipated that this research will lead to the eventual commercialization of a salt tolerant alfalfa that will provide increased profitability to the agriculture community dependent on forage production in environments where yields are limited by saline conditions. This objective should be completed by 2012. Objective 2. Characterize the physiological mechanism(s) of salinity tolerance in the selected alfalfa lines by identifying how the plants cope with saline soils. These characterizations will identify physiological mechanism(s) operational in the experimental salt-tolerant alfalfa lines. The results will provide critical guidelines for gene/pathway discovery in Objective 3. This objective should be completed by 2013. Objective 3. Identify genes and expression patterns associated with salt tolerance using oligonucleotide microarrays and cDNA amplified fragment length polymorphism (cDNA-AFLP). Determine functions and application of a subset of candidate genes in salinity tolerance. It is expected that lists of differentially expressed genes, including candidate genes described in the literature, will be compiled. We will also identify moderate numbers of cDNA-AFLP bands that are differentially expressed between salt tolerant and sensitive plants. A functional characterization of candidate genes identified by the microarray and cDNA-AFLP will be produced. This objective should be completed by 2013.

Présentation : USDA

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