Informations et ressources scientifiques
sur le développement des zones arides et semi-arides

Accueil du site → Projets de développement → Projets de recherche pour le Développement → 2011 → ENHANCED SUSTAINABILITY OF DRYLAND CROPPING SYSTEMS THROUGH CROP IMPROVEMENT

United States Department of Agriculture (USDA) 2011

ENHANCED SUSTAINABILITY OF DRYLAND CROPPING SYSTEMS THROUGH CROP IMPROVEMENT

Sustainability Dryland Cropping

United States Department of Agriculture (USDA) Research, Education & Economics Information System (REEIS)

Titre : ENHANCED SUSTAINABILITY OF DRYLAND CROPPING SYSTEMS THROUGH CROP IMPROVEMENT

Identification : WNP00663

Pays : Etats Unis

Durée : Jul 1, 2011 à Jun 30, 2016

Domaine : wheat rust ; cereal rust ; puccinia ; disease resistance ; rhizoctonia ; rnai ; effector protein ; camelina ; drought resistance ; fungal pathogen ; functional genomics ; host parasite interactions ; biofuel crop ; oilseed crop

Partenaire : WASHINGTON STATE UNIVERSITY 240 FRENCH ADMINISTRATION BLDG PULLMAN,WA 99164-0001

Objectifs
Four specific objectives fall under the main objective of improving the sustainability inland PNW cropping systems in the PNW : 1) Investigate an RNAi strategy for engineering durable resistance to rust and dissecting the genetics of virulence in the rust pathogen. 2) Cross genes conferring resistance or tolerance to Rhizoctonia solani from international wheat breeding programs into PNW wheat lines and map these genes. We will also determine if wheat genotype affects soil microbiology in a manner that can suppress this disease. 3) Cross genes conferring increased drought tolerance from international wheat breeding programs into PNW wheat lines and map these genes. 4) Develop and release Camelina lines that are well adapted to the low and intermediate rainfall regions of the PNW and tolerant to multiple group 2 herbicide chemistries.

Descriptif
We will look for essential stripe rust (P. striiformis) and stem rust (P. graminis) genes that can be used to engineer durable resistance by expressing their dsRNA in wheat lines. Genes expressed in haustoria will be cloned into VIGS vectors and delivered into wheat plants which will then be infected with rust to assay resistance. Genes that appear to have potential in VIGS assays will be used to make stable transgenic lines for verification of rust resistance. Drought tolerant germplasm from international programs will be crossed to PNW wheat lines and advanced lines will be used to create parents for breeding programs, map genes conferring drought tolerance and associate traits with increased tolerance to PNW drought conditions. Similar approaches will be used to transfer resistance to Rhizoctonia solani into PNW wheat varieties and map these genes. Potential synergies or relationships between the genes controlling root pathogens and drought resistance will be investigated. Camelina lines segregating for a variant ALS gene that confers resistance to group 2 herbicides will be advanced under selection to identify lines that perform well in low rainfall regions. Bulked seed from selected lines will be used in cropping experiments with and without group 2 herbicides to evaluate the utility of the mutant in field situations and to compare different cropping systems that incorporate Camelina.

Présentation : USDA

Page publiée le 10 octobre 2015, mise à jour le 12 novembre 2017