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United States Department of Agriculture (USDA) 2013

MOLECULAR MAPPING OF GENES FOR DROUGHT AND HEAT TOLERANCE IN COWPEA

Genes Drought Heat Cowpea

United States Department of Agriculture (USDA) Research, Education & Economics Information System (REEIS)

Titre : MOLECULAR MAPPING OF GENES FOR DROUGHT AND HEAT TOLERANCE IN COWPEA

Identification : TEX09569

Pays : Etats Unis

Durée : Dec 1, 2013 à Nov 30, 2016

Domaine : Plant Genetic Resources ; Plant Biological Efficiency and Abiotic Stresses Affecting Plants ; Plant Genome, Genetics, and Genetic Mechanisms ; Beans (dry)

Partenaire : TEXAS A&M UNIVERSITY 750 AGRONOMY RD STE 2701 COLLEGE STATION,TX 77843-0001

Objectifs
Drought and heat have been major production constraints in many crops for centuries. As the problems are becoming more serious and new powerful technologies are becoming available and more economical, there is a renewed worldwide interest in identification and incorporation of drought and heat tolerant genes in improved crop varieties. Cowpea, also known as blackeye pea in the USA, could be an excellent system and a source of genes to address the issue of plant drought and heat tolerance due to the availability of genetic resources unique in these stress tolerances and genetics. The goal of this proposal is to take the advantage of recently-developed next generation high-throughput sequencing technology-based Restriction site-Associated DNA sequencing (RAD-seq) to rapidly map the genes controlling drought and heat tolerance in cowpea and develop SNP (single nucleotide polymorphism) markers enabling efficient genetic manipulation and breeding of the traits within cowpea and potentially in other related crop species. Toward this end, we have developed a mapping population of 110 recombinant inbred lines (RILs) (F2:6) from a cross of two cowpea lines (IT98K-476-8 and GEC) that are highly tolerant or susceptible to both drought and high temperature (see below). Currently, we are augmenting the population into more than 200 RILs which will be available before this project commences (hereafter the population is defined as the ITG RIL population). In this research, we will especially accomplish the following three specific research objectives : Phenotypically analyze the drought and heat tolerances of the ITG RIL population ; Genetically map the genes controlling the drought and heat tolerances using RAD-seq ; and Identify SNP markers closely linked to the genes of drought and heat tolerances, and develop molecular tools enabling enhanced breeding of the traits in cowpea and related species

Présentation : USDA

Page publiée le 31 octobre 2015, mise à jour le 22 novembre 2017