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United States Department of Agriculture (USDA) 2000

COMPARATIVE ECOLOGY OF DIVERSE NEMATODE SPECIES SETS IN XERIC RHIZOSPHERE ECOSYSTEMS

Nematode Xeric Rhizosphere

United States Department of Agriculture (USDA) Research, Education & Economics Information System (REEIS)

Titre : COMPARATIVE ECOLOGY OF DIVERSE NEMATODE SPECIES SETS IN XERIC RHIZOSPHERE ECOSYSTEMS

Identification : CA-R*-NEM-6869-H

Pays : Etats Unis

Durée : Nov 1, 2000 à Sep 30, 2005

Mots clés : nematode ecology rhizosphere feeding dispersal nematode biology reproduction biodiversity nematode population population genetics polymerase chain reaction comparative analysis soil management pollution control integrated pest management ecosystems life history environmental factors phylogenetics animal morphology motility

Partenaire : UNIVERSITY OF CALIFORNIA RIVERSIDE,CA 92521

Objectifs
The immediate purpose of this research program would be to apply both new and traditional methods to the ecological characterisation of a taxonomically diverse range of carefully selected nematode species from rhizospheres of wild and cultivated plants, with the aim of accurately characterising those aspects of greatest relevance to theory and application of holistic analyses. These are 1) feeding 2) dispersal, and 3) reproduction

Descriptif
To establish and maintain coherence in our approaches and sampled environments, we will focus primarily on nematodes obtained from xeric rhizosphere habitats. Not only will this allow us to maintain focus and circumscription with regards to the ecosystems investigated, but it will also : 1. Be of greatest direct relevance to the natural and agricultural ecosystems surrounding UC-Riverside. 2. Allow us to focus on habitats where natural inputs of energy, water and organic matter are relatively easy to pinpoint in time and space. 3. Allow us to test the assumptions of holistic approaches under different conditions than the temperate ecosystems in which such approaches were invariably developed. The employed methods will rely on a combination of four methodologies : 1. Establishment of and experimentation with nematode cultures, in order to determine life history characteristics under controlled conditions. 2. Direct sampling and characterisation of field populations, in order to obtain data on population genetics, environmental conditions and biotic associates in natural conditions. 3. PCR-based molecular protocols allowing analysis of individual nematodes, to determine their taxonomic identities and phylogenetic affinities, as well as providing information on their population genetics. 4. VCE-based observation and recording of morphology (for identification) and motility (for measurement of various ecological properties).

Présentation : USDA

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