Informations et ressources scientifiques
sur le développement des zones arides et semi-arides

Accueil du site → Master → Etats Unis → 1979 → A starch gel electrophoresis study of several populations of the saguaro cactus (Carnegiea gigantea)

University of Arizona (1979)

A starch gel electrophoresis study of several populations of the saguaro cactus (Carnegiea gigantea)

Jurgenson, Dorothea Ells

Titre : A starch gel electrophoresis study of several populations of the saguaro cactus (Carnegiea gigantea)

Auteur : Jurgenson, Dorothea Ells

Université de soutenance : University of Arizona

Grade : Master of Science (MS) 1979

Résumé
In this study, starch gel electrophoresis was used to identify inter- and intra-populational allele frequency differences in the sagua ro » Carnegiea gigantea Engelnu (Britt, and Rose). Seeds were taken from adult plants at six sites representing different environmental conditions ; adults sampled at each site were divided into three broad age groups based on size. The seeds were germinated and 3-8 week old seedlings used in electrophoretic studies. Thirteen enzyme systems were examined, of which seven gave clear enough results for further study. Assignment of isozyme hands to loci and alleles was based on analysis of progeny from a cross between two specific adults. Thirteen loci were identified of which three, an esterase, a glutamic oxaloacetate transaminase and a phosphoglucose isomerase were polymorphic. Each polymorphic locus was controlled by two alleles in all populations except that the esterase showed a third allele in low frequency for one population. Statistical analysis of allele frequencies showed no consistent intra-population differences. Inter-population differences were sig nificant and were analyzed by computer against several defined environ mental factors. Particularly strong correlation was found between degree of drought stress and allele frequency variation. This and other correlated factors were interpreted as relevant to saguaro establishment and survival.

Mots clés : Saguaro — Genetics.

Présentation

Version intégrale (1,40 Mb)

Page publiée le 12 novembre 2015, mise à jour le 18 décembre 2017