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University of Arizona (1983)

THE CHARACTERIZATION OF PHOSPHOLIPIDS FROM THREE XEROPHYTIC CUCURBITS

Goodman, Lorie Ann

Titre : THE CHARACTERIZATION OF PHOSPHOLIPIDS FROM THREE XEROPHYTIC CUCURBITS.

Auteur : Goodman, Lorie Ann

Université de soutenance : University of Arizona

Grade : Master of Science (MS) 1983

Résumé
Analysis of the phospholipids from three species of Cucur bitacea (Cucurbita foetidissima, Cucurbita digitata, and Apodanthera undulata) was done to determine their possibility for lecithin manu facture. The phospholipids were isolated using silicic acid chroma tography and quantitated by a colorimetric analysis. The component phosphatides were separated using thin-layer chromatography. All three species contained phosphatidylcholine, phosphatidylethanola mine and phosphatidylinositol as their major constituent phospho lipids. Fatty acid analysis by gas-liquid’ chromatography (GLC) revealed that the major fatty acid in the three cucurbits was lino leic and the minor one, myristic. GLC analysis of each component phosphatide indicated that there was considerable variation in their distribution of fatty acids. The presence of small amounts of con jugated fatty acids in the three species’ phosphatides was deter mined using ultraviolet spectrophotometry. All results indicated that a good quality lecithin could be produced from these three Cucurbitaceae oils.

Mots clés : Cucurbita. ; Lecithin. ; Phospholipids.

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Page publiée le 4 février 2016, mise à jour le 16 février 2018