Informations et ressources scientifiques
sur le développement des zones arides et semi-arides

Accueil du site → Master → Etats Unis → 1988 → Gene expression in two different genotypes of alfalfa under salt stressed and unstressed conditions

University of Arizona (1988)

Gene expression in two different genotypes of alfalfa under salt stressed and unstressed conditions

Zheng, Liansheng

Titre : Gene expression in two different genotypes of alfalfa under salt stressed and unstressed conditions

Auteur : Zheng, Liansheng

Université de soutenance : University of Arizona

Grade : Master of Science (MS) 1988

Résumé
Gene expression in two different genotypes of alfalfa, salt-tolerant and salt-sensitive, was examined by studying differences in protein products coded for by poly(A+) RNA isolated from shoot and root tissue. Plants were grown in hydroponics under unstressed or salt-stressed conditions. Two salinity levels (low salt : 30 mM NaCl and 6 mM CaCl2 and high salt : 133 mM NaCl and 27 mM CaCl2) and one unstressed control were applied. The salt-tolerant genotype showed higher biomass accumulation than the salt-sensitive genotype under both control and salt-stressed conditions. The difference in biomass accumulation between the two genotypes was greatest at the highest salt level. The effect of salt stress on gene expression was studied via in vitro translation of poly (A+) RNA with (35S) -methionine. The labeling pattern was similar in all treatments when analyzed by one dimensional SDS-PAGE. However, a two dimensional analysis (isoelectric focusing followed by SDS-PAGE) showed that salt-stress induced a number of new proteins and repressed several others.

Mots clés : Alfalfa — Genetics. ; Plants — Effect of salts on. ; Plant molecular genetics.

Présentation

Version intégrale (2,22 Mb)

Page publiée le 3 février 2016, mise à jour le 20 février 2018