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Universidade Estadual da Paraíba - UEPB (2010)

Ciclos de vidas comparados e variabilidade genética de Aedes aegypti (DIPTERA : CULICIDAE) do semi-árido paraibano

Castro Júnior Francisco Pires de

Titre : Ciclos de vidas comparados e variabilidade genética de Aedes aegypti (DIPTERA : CULICIDAE) do semi-árido paraibano

COMPARED LIFE CYCLES AND GENETIC VARIABILITY OF Aedes aegypti (DIPTERA : CULICIDAE) IN PARAIBA SEMI-ARID

Auteur : Castro Júnior Francisco Pires de

Université de soutenance : Universidade Estadual da Paraíba - UEPB

Grade : Mestre 2010

Résumé
The efficiency of a program to control Aedes aegypti depends on the knowledge about the biological and genetic variations that occur between their populations. The present study addressed to compare the life cycles patterns and genetic variability among populations of A. aegypti collected in different regions of Paraiba semi-arid. Life cycles were studied at environmental temperature (26 ± 2ºC, relative humidity of 60 ± 10% and photophase of 12 hours), the period of development and viability of egg phases, larva and pupa, sex ratio, longevity, fecundity and size of adults were evaluated daily. Beyond these variables grouping analysis (Cluster Analysis), using a matrix of Euclidean distance by the method of unweighted average was used. The Analysis of gene structure of populations was carried out by total DNA extractions and RAPD-PCR analysis. Phase’s durations of egg, larva and pupa varied from 3.79 to 4.79 days, 9.15 to 10.89 days and 2.18 to 2.59 days, respectively. Adults’ longevity of A. aegypti means variation from 37.82 to 58.29 days for males and from 40.03 to 73.07 days for females. Populations of A. aegypti from Serra Branca and Cuité cities presented major similarity in relation to the formed groupings. Genetic variability indexes showed highest diversity in Barra de Santana population (P = 93.33%, H = 0.373), however, it was lowest in Cuité population (P = 60.00%, H o o = 0.171). According to the results it was verified that there is a distinct pattern of development among populations of A. aegypti from different municipalities of Paraíba semi-arid. Genetic diversity heterozygosity H and polymorphisms indexes suggest a high intrapopulation genetic variation and low interpopulation variability. Such fact may indicate constant migration of the vectors to these locations with high number of individuals.

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Page publiée le 28 février 2016, mise à jour le 19 juillet 2018