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King Abdulaziz University (2014)

Detection of Aflatoxin M1 in Camel Milk in Jeddah Province

Al-Kenani, Amani Ali

Titre : Detection of Aflatoxin M1 in Camel Milk in Jeddah Province

تقدير الافلاتوكسين م1 في ألبان الجمال في محافظه جده

Auteur : Al-Kenani, Amani Ali

Université de soutenance : King Abdulaziz University

Grade : Master of Science (MS) 2014

Milk samples may be contaminated with mycotoxins which is dangerous health problems. Milk samples (160 samples) of four different species of mammals including camel, cow goat and sheep milk samples in addition to sterilized milk samples were collected from different farms of Mecca, Jeddah Rode, Hada Al-Sham Farm and supermarkets in Jeddah. All of the samples were examined for the presence of aflatoxin M1 (AFM1) using by immune affinity column and a flourometer. To our knowletage, occurrence of AFM1 in different dairies milk is presented for the first time in Saudi Arabia. Analytical results showed that 58.12% of the samples were contaminated with AFM1 ; 38.75% being above the maximum authorized level (0.05 µgl-1) set by European regulations for AFM1 in milk by average concentration of 0.045 µgl-1. The incidence rates of AFM1 in camel, cow, goat, sheep and pasteurized milks were 34.4%, 81.25%, 65.52%, 53.12%, and 56.25%, respectively. Fungal association and mycotoxin analysis of 20 samples of feedstuffs (alfalfa, hay, barley, acacia, corn and wheat), collected from various animal farms and vendor were determined. Total counts of Aspergillus, Penicillium and Fusarium in the animal feed samples were 25.42-22.91 ×104 and 8.48-5.96 ×104 and 12.6-11.6 ×104 respectively. All 31 Aspergillus isolates were screened for aflatoxin and ochratoxin production. Thirty isolates of the genus Aspergillus produced mycotoxins and they were Aspergillus flavus, A. niger, A. ochraceus and A. parasiticus. Each isolate can produce one or two types of mycotoxins, which were separated and identified using thin-layer chromatography (TLC). The detected toxins were Aflatoxin B1, Aflatoxin B2 and Ochratoxin A. In conclusion, feed samples were contaminated with toxigenic fungi which were responsible for the presence of aflatoxin M1 in milk.


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