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University of Khartoum (2015)

In Vitro Culture of Zygotic Embryos of Date Palm (Phoenix dactylifera L.).

Abdalla, Mohanned Abdalla Ali

Titre : In Vitro Culture of Zygotic Embryos of Date Palm (Phoenix dactylifera L.).

Auteur : Abdalla, Mohanned Abdalla Ali

Université de soutenance : University of Khartoum

Grade : Master of Science (MS) 2015

Résumé
In Vitro Culture of Zygotic Embryos of Date Palm (Phoenix dactylifera L. M.Sc. (Horticulture) Mohanned Abdalla Ali Aballa Abstract : the objective of this study was to evaluate the morphogenic responses of the in vitro cultured embryos of two date palm (Phoenix dactylifera L.) cultivars “Barakawi” and “Mishrigi Wad Laggai” to eight seed water-soaking treatment. A completely randomized design was used, with three replicates and 20 embryos per replicate. Cultures were initiated on modified media containing MS basal salts supplemented with (per liter) : 30 g sucrose ; 100 mg I-inositol ; 0.4 mg thiamin ; 100 mg 2,4-dichlorophenoxyacetic acid (2,4-D) and 7.0 g Phytagar. Several types of responses were obtained within 6 to 13 weeks from culture initiation, including formation of callus, germination into whole seedlings and adventitious root formation in the preformed callus. The magnitude and type of response varied with cultivar and water soaking treatment, ranging from recalcitrant to 100% response. All water soaking treatments increased morphogenic responses relative to the control, with the highest values obtained by the 6-day soaking treatment. The response competence generally diminished with extending the soaking treatment, regardless of cultivar. High values of morphogenic responses were obtained with “Mishrigi Wad Laggai” compared with “Barakawi”. The results suggest soaking of date palm seeds in water for 6 days prior to embryo excision and transplanting as a pretreatment for accelerating callus induction and morphogenesis

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Page publiée le 14 septembre 2016, mise à jour le 11 août 2018