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Jordan University of Science and Technology (JUST) 2002

Comparative Responses of Tomato Lycopersicon esculentum Mill MicroshootCallus Cell Suspension and Hydroponic Cultures to Salinity

Aktham Abdel Aziz Rashad Abu-Khadijeh

Titre : Comparative Responses of Tomato Lycopersicon esculentum Mill MicroshootCallus Cell Suspension and Hydroponic Cultures to Salinity

Auteur : Aktham Abdel Aziz Rashad Abu-Khadijeh

Université de soutenance : Jordan University of Science and Technology (JUST)

Grade : Master of Science (MS) 2002

Résumé
The use of microshoots, callus, cell suspension and hydroponic cultures to evaluate salt stress responses in two (JO112 and JO992) tomato (Lycopersicon esculentum Mill.) landraces was determined. Microshoots, callus, suspension cells and hydroponic culture were grown on media supplemented with 0,50,100,150 and 200 mM NaCl and CaCl2 in equal molar ratio, growth and physiological traits were determined. Most microshoots of the two landraces did not develop roots when exposed to 150 mM or higher salt levels, and negative response was happened in growth parameters (FW, DW and shoot height). Callus growth, packed cell volume, and cell growth (FW and DW) were reduced significantly with elevated salt level. Similar growth responses were obtained in in vivo plants grown in hydroponic system. Na+, Cl¯ and Ca+² concentrations were increased in microshoots, callus and hydroponic grown plants with increased salinity in both genotypes. On the other hand, K+ content was decreased with increased salinity. Proline accumulation was increased with salinity in the microshoots, callus, suspended cells and hydroponic grown plants. The effect of salinity treatments on polypeptide levels in tomato microshoots was analyzed using sodium dodecyl sulfate – polyacrylamide gel electrophoresis SDS-PAGE. The salt-induced polypeptides 18, 26 and 41 KDa, and the salt-depressed 33 KDa were obtained in salt stressed tomato microshoots compared to the control.

Présentation

Page publiée le 2 novembre 2016, mise à jour le 22 mars 2019