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King Abdulaziz University (2016)

Molecular genetic markers for drought tolerance in some barley (Hordeum vulgare L.) cultivars

Al-aboud, Norah Mohammed

Titre : Molecular genetic markers for drought tolerance in some barley (Hordeum vulgare L.) cultivars

Auteur  : Al-aboud, Norah Mohammed

Université de soutenance : King Abdulaziz University

Grade : Doctorate Thesis 2016

Résumé
Barley (Hordeum vulgare L.) is more tolerant of drought, low soil fertility and soil salinity than other major cereals, so it is particularly important in regions where climatic conditions are unsuitable for other temperate cereals (and other crops) to be grown as an economically viable crop. From seven barley genotypes (Giza126, Giza132, Giza2000, El-Qassim, El-Taif, Hail and Erginel-90), two were selected as the most drought tolerant (El-Qassim) and the sensitive (Erginel-90) genotypes on the basis of their performance for germination percentage, eight yield-related and relative water content (RWC) percentage. They were evaluated along with their F1 and F2 for their relation to water stress. Bulked segregate analysis was used to analyze DNA extracts with RAPD-PCR and ISSR-PCR techniques for the two contrasting parents, their F1 and the two extremes of F2 groups (drought tolerant and drought sensitive). Eight RAPD primers revealed a total of 69 amplified products, whereas 49 fragments were polymorphic percentage (71%). Out of 8 RAPD primers Three primers gave four specific positive markers, two primer detected three specific marker for drought tolerance (OPA-07640bp, OPA-07415bp , OPA-17655bp and OPB-17655bp) and one primer detected one specific marker (OPB-14910bp) for drought-sensitivity. Eleven ISSR primers revealed a total of 78 amplified products, whereas 64 fragments were polymorphic percentage (82%). Out of 11 ISSR primers only four primers (HB-08, TA-01,TA-02 and 844B) developed six positive markers (HB-08735bp, HB-08545bp, TA-01550bp, TA-02755bp, TA-02175bp and 844B175bp) for drought whether tolerant or susceptible in barely. The present study indicated that RAPD and ISSR markers, combined with bulked segregant analysis (BSA), could be used to identify molecular markers as indicators for drought tolerance in barley. Once these markers are identified, they can be used in barley breeding programs as a selection tool in early generations. Key words : Barley (Hordeum vulgare L.), drought, molecular markers, RAPD, ISSR

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Page publiée le 7 septembre 2016, mise à jour le 18 janvier 2019