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University of Tsukuba (2014)

Genetic Analysis of Salt Tolerance in Asian Germplasm of Barley

SBEI Hanen

Titre : Genetic Analysis of Salt Tolerance in Asian Germplasm of Barley

Auteur : SBEI Hanen

Université de soutenance : University of Tsukuba

Grade : Doctor of Philosophy (PhD) 2014

Présentation
In this study we explore the natural variation of barley using a large number of accessions to determine key traits for salt tolerance in barley. Association mapping method is used as a new tool to identify QTLs for salt tolerance in barley.
The overall objectives of the present research are : 1. To understand key traits determining salt tolerance at seedling stage ; 2. To provide new tools to better exploit the genetic variation of salt tolerance ; 3. To evaluate genetic variation in salt tolerance of Asian barley collection ; 4. To identify new QTL associated with salt tolerance.
In the Chapter 1, the literature review devised on 3 parts including the importance of barley in the word, salt stress mechanisms and breeding for salt tolerance in barley. Also, we present the problematic of the research study and the thesis outline to achieve our objectives.
In the Chapter 2, the author evaluated a set of 296 accessions of Asian barley for salt tolerance with 250 mM NaCl in a hydroponic solution. The objectives were to evaluate the genetic variation in salt tolerance of barley and to determine the suitable traits for salt tolerance assessement at seedling stage in barley accessions.
In the Chapter 3, the author analyse the association of salt tolerance with 384 SNP markers to identify QTLs for salt tolerance. The objectives were to determine the population structure of the Asian barley accessions and to identify SNP markers associated with salt tolerance at seedling stage, based on the association analysis.
In the Chapter 4, the author discussed the findings presented in this thesis in relation to the current status and the prospects of breeding for salt tolerance in barley and cereals. The impact of our results on major issues related to trait discovery strategies for salt tolerance. These include phenotyping strategies and association mapping in breeding for salt stress tolerance.

Présentation

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