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University of Tsukuba (2014)

Mapping and Analysis of QTLs for Tolerance to Salinity in Durum Wheat


Titre : Mapping and Analysis of QTLs for Tolerance to Salinity in Durum Wheat

Auteur : TURKI Nejla

Université de soutenance : University of Tsukuba

Grade : Doctor of Philosophy (PhD) 2014

The research presented in this thesis aims to identify traits and QTLs that underlie salinity tolerance in durum wheat. The objectives are :
1. To determine key traits associated with salinity tolerance and parameters for the assessment of salinity tolerance ;
2. To evaluate variation of salinity tolerance in a worldwide durum wheat collections.
3. To identify QTLs associated with salinity tolerance at the seedling and maturity stages.
In Chapter 2 the response of 119 varieties of durum wheat to salinity stress was evaluated at seedling and maturity stages. At seedling stage, ten agronomic traits were recorded and four traits at maturity stage were measured. The wide variation of several traits under salinity stress showed the importance of these traits in salinity tolerance. Genetic correlation was calculated in term to understand the relationship between each trait. This study allowed the choice of key trait as a parameter for the assessment of salinity tolerance in durum wheat. Chapter 3 describes the evaluation of the variation in salinity tolerance in worldwide durum varieties. An association mapping approach is utilized to identify promising alleles contributing to salinity tolerance to facilitate future breeding for salinity tolerance.

In Chapter 4, genetic analysis of the variation observed in salinity tolerance using Saragolla×Razzak (F2:3) mapping population is described. Saragolla and Razzek were chosen as parents based on the differences in salinity tolerance between these cultivars. The General Discussion in Chapter 5 discusses the findings presented in this thesis in relation to the current status and the prospects of breeding for salinity tolerance in durum wheat. The impact of our results on major issues related to trait discovery strategies for salinity tolerance and salinity tolerance mechanisms in durum wheat and other related crops are addressed.


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